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人类免疫缺陷病毒1 Gag-特异性T淋巴细胞增殖反应特征的研究

Characterization of HIV-1 Gag-specific proliferative T cell responses in Chinese HIV-1 infected patients
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摘要 目的了解人类免疫缺陷病毒(HIV)感染者HIV-1Gag-特异性T淋巴细胞增殖反应特征,分析它们与疾病进展的关系,探讨HIV特异性免疫功能缺陷的发生机制。方法采用BrdU掺入的流式细胞仪胞内染色法,检测34例HIV-1感染者PBMC对HIV-1Gag抗原的特异性增殖反应频率,分析它们与CD4+T细胞绝对值、血浆病毒载量及CD8+T细胞活化指标的相关性。结果HIV感染者HIV-1Gag-特异性CD4+T细胞增殖率显著低于健康对照组(P<0·01),与CD8+T细胞CD38表达负相关(P<0·01),与CD4绝对值正相关(P<0·01),与血浆病毒载量负相关(P<0·05);中国HIV感染者HIV-1Gag-特异性CD8+T细胞增殖率显著高于健康对照组(P<0·01),与CD8+T细胞CD38表达正相关(P<0·01),与CD4绝对值显著负相关(P<0·01),与血浆病毒载量不相关(P>0·05)。结论HIV感染者T细胞增殖功能异常,HIV-1Gag-特异性CD4+T细胞增殖功能减低,其程度与疾病进展密切相关。 Objective To investigate the characteristics of HIV-1 Gag-specific T lymphocyte proliferative responses in Chinese HIV infected patients, analyze their associations with disease progression and explore the mechanism of the HIV-specific immune dysfunction in HIV infection. Methods Specific proliferative responses of PBMC from thirty four HIV-1 infected patients to HIV-1 Gag antigen were detected by a BrdU incorporated flow cytometry intracellular stain, their relations with CD4^ + T cells absolute counts, plasma viral loads and CD8^ + T cell activate status were analyzed. Results HIV-1 Gag-specific CD4^ + T cell proliferative responses of HIV-infected patients were significantly lower than that of healthy controls ( P 〈 0. 01 ), correlated inversely to CD38 expression on CD8^ + T lymphocytes and plasma viral loads ( P 〈 0. 05, P〈0. 05), and directly to CD4^+T cell absolute counts (P 〈 0. 01 ). HIV-1 Gag-specific CD8^+T cell proliferative responses of HIV-infected patients were significantly higher than that of healthy controls ( P 〈 0. 01 ), correlated directly to CD38 expression on CD8 ^+ T lymphocytes ( P 〈 0. 05 ) , and inversely to Cd4^+ T cell absolute counts(P〈0. 01), but not correlated to viral loads (P 〉0. 05). Conclusion HIV-1 Gagspecific T cell proliferative response were abnormal in Chinese HIV-1 infected persons, HIV-1 Gag-specific CD4^ + T cell proliferative responses were decreased and their frequencies were closely correlated with disease progression.
出处 《中华医学杂志》 CAS CSCD 北大核心 2006年第44期3099-3103,共5页 National Medical Journal of China
基金 国家"十五"科技攻关课题资助项目(2004BA719A12) 国家自然科学基金资助项目(30471548) 科技部重大基础研究前期研究专项基金资助项目(2004CCA02000)
关键词 HIV-1 CD4阳性T淋巴细胞 抗原 CD8 HIV-1 CIM-positive T-lymphocytes Antigens, CD8
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参考文献26

  • 1Kalams SA,Walker BD.The critical need for CD4 help in maintaining effective cytotoxic T lymphocyte responses.J Exp Med,1998,188:2199-2204.
  • 2Oxenius A,Price DA,Easterbrook PJ,et al.Early highly active antiretroviral therapy for acute HIV-1 infection preserves immune function of CD8 + T lymphocytes.Proc Natl Aced Sci USA,2000,97:3382-3387.
  • 3Matloubian M,Concepcion RJ,Ahmed R.CD4 + T cells are required to sustain CD8 + cytotoxic T-cell responses during chronic viral infection.J Virol,1994,68:8056-8063.
  • 4Lu W,Achour A,Arlic M,et al.Enhanced dendritic cell-driven proliferation and anti-HIV activity of CD8 (+) T cells by a new phenothiazine derivative,aminoperazine.J Immunol,2001,167:2929 -2935.
  • 5Musey LK,Krieger JN,Hughes JP,et al.Early and persistent human immunodeficiency virus type 1 (HIV-1)-specific T helper dysfunction in blood and lymph nodes following acute HIV-1infection.J Infect Dis,1999,180:278-284.
  • 6李太生,邱志峰,王爱霞,盛瑞媛.HIV感染和AIDS患者T淋巴细胞免疫病理改变的研究[J].中华医学杂志,2002,82(20):1391-1395. 被引量:21
  • 7张宏伟,邱志峰,李太生.HIV感染长期不进展者与艾滋病患者HIV-1 gag特异CD_8^+T细胞应答[J].中华内科杂志,2004,43(12):911-914. 被引量:5
  • 8Boritz E,Palmer BE,Livingston B,et al.Diverse repertoire of HIV-1 P24-specific,IFN-γ producing CD4 + T cell clones following immune reconstitution on highly active antiretroviral therapy.J Immunol,2003,170:106-116.
  • 9Finkel TH,Banda NK.Indirect mechanisms of HIV pathogenesis:how does HIV kill T cells? Curr Opin Immunol,1994,6:605-615.
  • 10Haase AT.Population biology of HIV-1 infection:viral and CD4 + T cell demographics and dynamics in lymphatic tissues.Annu Rev Immunol,1999,17:625-656.

二级参考文献23

  • 1Ullum H, Lepri AC, Victor J, et al. Increased losses of CD4+CD45RA+ cells in late stages of HIV infection is related to increased risk of death: evidence from a cohort of 347 HIV-infected individuals. AIDS,1997, 11: 1479-1485.
  • 2June CH, Bluestone JA, Nadler LM, et al. The B7 and CD28 receptor families. Immunol Today, 1994, 15:321-331.
  • 3Brinchmann JE, Dobloug JH, Heger BH, et al. Expression of costimulatory molecule CD28 on T cells in human immunodeficiency virus type 1 infection: functional and clinical correlations. J Infect Dis, 1994,169:730-738.
  • 4Funaro A, Spagnoli GC, Ausiello CM, et al. Involvement of the multilineage CD38 molecule in a unique pathway of cell activation and proliferation. J Immunol, 1990, 15: 2390- 2396.
  • 5Levacher M, Hulstaert F, Tallet S, et al. The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value. Clin Exp Immunol, 1992, 90: 376-382.
  • 6Giorgi JV, Liu Z, Hultin LE, et al. Elevated levels of CD38+CD8+T cells in HIV infection add to the prognostic value of low CD+ T cells levels: results of 6 years of follow-up. The Los Angeles Center, Multicenter AIDS Cohort Study. J Acquir Immune Defic Syndr, 1993, 6:904-912.
  • 7Brinchmann JE. Differential responses of T cell subsets: possible role in the immunopathogenesis of AIDS. AIDS, 2000,14:1689-1700.
  • 8Autran B, Carcelain G,Li TS ,et al. Positive effects of combined antiretroviral therapy on CD4+ T cell homeostasis and function in advanced HIV disease. Science, 1997, 277:112-116.
  • 91993 revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. MMWR Recomm Rep, 1992,41(RR-17): 1-19.
  • 10Lang W, Perkins H, Anderson RE, et al. Patterns of T lymphocyte changes with human immunodeficiency virus infection: from seroconversion to the development of AIDS. J Acquir Immune Defic Syndr, 1989, 2: 63-69.

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