摘要
目的了解人类免疫缺陷病毒(HIV)感染者HIV-1Gag-特异性T淋巴细胞增殖反应特征,分析它们与疾病进展的关系,探讨HIV特异性免疫功能缺陷的发生机制。方法采用BrdU掺入的流式细胞仪胞内染色法,检测34例HIV-1感染者PBMC对HIV-1Gag抗原的特异性增殖反应频率,分析它们与CD4+T细胞绝对值、血浆病毒载量及CD8+T细胞活化指标的相关性。结果HIV感染者HIV-1Gag-特异性CD4+T细胞增殖率显著低于健康对照组(P<0·01),与CD8+T细胞CD38表达负相关(P<0·01),与CD4绝对值正相关(P<0·01),与血浆病毒载量负相关(P<0·05);中国HIV感染者HIV-1Gag-特异性CD8+T细胞增殖率显著高于健康对照组(P<0·01),与CD8+T细胞CD38表达正相关(P<0·01),与CD4绝对值显著负相关(P<0·01),与血浆病毒载量不相关(P>0·05)。结论HIV感染者T细胞增殖功能异常,HIV-1Gag-特异性CD4+T细胞增殖功能减低,其程度与疾病进展密切相关。
Objective To investigate the characteristics of HIV-1 Gag-specific T lymphocyte proliferative responses in Chinese HIV infected patients, analyze their associations with disease progression and explore the mechanism of the HIV-specific immune dysfunction in HIV infection. Methods Specific proliferative responses of PBMC from thirty four HIV-1 infected patients to HIV-1 Gag antigen were detected by a BrdU incorporated flow cytometry intracellular stain, their relations with CD4^ + T cells absolute counts, plasma viral loads and CD8^ + T cell activate status were analyzed. Results HIV-1 Gag-specific CD4^ + T cell proliferative responses of HIV-infected patients were significantly lower than that of healthy controls ( P 〈 0. 01 ), correlated inversely to CD38 expression on CD8^ + T lymphocytes and plasma viral loads ( P 〈 0. 05, P〈0. 05), and directly to CD4^+T cell absolute counts (P 〈 0. 01 ). HIV-1 Gag-specific CD8^+T cell proliferative responses of HIV-infected patients were significantly higher than that of healthy controls ( P 〈 0. 01 ), correlated directly to CD38 expression on CD8 ^+ T lymphocytes ( P 〈 0. 05 ) , and inversely to Cd4^+ T cell absolute counts(P〈0. 01), but not correlated to viral loads (P 〉0. 05). Conclusion HIV-1 Gagspecific T cell proliferative response were abnormal in Chinese HIV-1 infected persons, HIV-1 Gag-specific CD4^ + T cell proliferative responses were decreased and their frequencies were closely correlated with disease progression.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第44期3099-3103,共5页
National Medical Journal of China
基金
国家"十五"科技攻关课题资助项目(2004BA719A12)
国家自然科学基金资助项目(30471548)
科技部重大基础研究前期研究专项基金资助项目(2004CCA02000)