基质金属蛋白酶3和白细胞介素1在突出的腰椎间盘组织中的含量及其相关性研究

DETERMINATION ON CONTENTS OF MATRIX METALLOPROTEINASE 3 AND INTERLEUKIN 1 IN TISSUES OF LUMBAR DISC HERNIATION AND THEIR ROLES IN PATHOGENESIS

目的检测正常和突出的腰椎间盘组织中的基质金属蛋白酶3(m atrix m eta lloprote inase 3,MM P-3)、白细胞介素1(in terleuk in 1,IL-1)含量,探讨MM P-3及IL-1间的关系,以及在椎间盘突出症中的作用。方法检测标本分为实验组和对照组,为2003年6月~2004年12月突出的腰椎间盘标本,实验组30例为膨隆型11例,突出型9例,脱垂游离型10例。其中男14例,女16例。年龄33~64岁。对照组9例,为非突出的腰椎间盘标本。其中男4例,女5例。年龄21~58岁。用酶联免疫吸附实验方法测定两组椎间盘标本中MM P-3、IL-1的含量。结果实验组MM P-3膨隆型、突出型和脱垂游离型分别为14.25±1.32、19.89±2.97和20.69±2.18 ng/m l,IL-1膨隆型、突出型和脱垂游离型分别为8.52±0.22、11.88±0.52和11.90±0.73 pg/m l,含量明显高于对照组(P<0.01);实验组中突出型和脱垂游离型MM P-3、IL-1的含量差异无统计学意义(P>0.05),明显高于膨隆型(P<0.01);对照组和各型突出的腰椎间盘标本中MM P-3、IL-1含量差异有统计学意义(P<0.01)。结论退行性变的腰椎间盘能产生MM P-3及IL-1,且二者在椎间盘退行性变中起着重要作用。

Objective To determine the contents of matrix metalloproteinase 3 （MMP-3） and interleukin 1 （IL-1） in the tissues of the lumbar disc herniation and to investigate their roles in the pathogenesis. Methods The tissues of the herniated lumbar disc were obtained from 30 patients undergoing surgery for persistent radiculopathy from June 2003 to December 2004 and at the same time these samples were divided into the following three experimental groups： the bulge group （n=11）, the protrusion group （n=9）, and the prolapsus group （n= 10）,14 males, 16 females, aged 33-64 years. As the control group, 9 lumbar disc specimens were harvested from 9 patients（4 males, 5 females, aged 21-58 years） suffering from bursting fracture of the lumbar spine. The specimens were analyzed by the ELISA method for the contents of MMP-3 and IL-1. Results The contents of MMP 3 （14.25 4±1.32, 19.89±2.97, 20.69±2.18 ng/ml in the bulge group, protrusion group and prolapsus group, separately） and IL-1 （8.52±0.22, 11.88±0.52, 11.90±0.73 pg/ml in the bulge group, protrusion group and prolapsus group, separately） in the experimental groups were significantly higher than those in the control group. The contents of MMP-3 and IL-1 in the protrusion group were not significantly higher than those in the prolapsus group, but they were significantly higher than those in the bulge group（P^0.01）. The contents of MMP 3 had asignificant relationship with the contents of IL 1 in the three experimental groups and the control group（P〈0.01） the lumbar disc herniation can produce both MMP-3 relationship with each other.