摘要
在4～35℃温度范围内，采用酶法成功地从制革固体废弃物中提取胶原，探讨了试验原理及方法，确定了最佳工艺条件：Ca（OH）2浓度的质量分数4．6％、酶浓度的质量分数2‰、酶解温度30℃、反应时间72h，并对所提取的胶原进行了电泳、红外光谱的表征。
The collagen was extracted successfully from solid waste of leather industry under 4 - 35℃. The principle and method of extraction was investigated. The optimal extraction condition is confirmed： Ca（OH） 2 concentration is 4.6%, enzyme concentration is 2‰, temperature is 30℃ and time is 72h. The extracted collagen was characterized by SDS - PAGE and IR spectrum.
出处
《中国皮革》
CAS
北大核心
2006年第23期15-17,共3页
China Leather
基金
北京市教委资助项目KM:20041000519
关键词
胶原
胃蛋白酶
水解
提取
ollagen
pepsin
hydrolyze
extract
