复方白花龙胆中白花龙胆的鉴别和甘草酸的含量测定

Identification of Gentiana purdomii Marq. by TLC and content determination of glycyrrhizic acid by RP-HPLC in Compound Baihualongdan

目的 建立复方白花龙胆的质量标准。方法 采用TLC法定性，RP—HPLC法测定甘草中甘草酸的含量。用Kromasil C18色谱柱（250mm×4．6mm，5μm），以甲醇-0．2mol·L^-1。醋酸铵-冰醋酸（67：33：1）为流动相，流速1．0ml·min^-1，柱温25℃，检测波长250nm.结果 鉴别了复方中的白花龙胆；甘草酸铵进样量在0．402—2．010μg之间，进样量与峰面积呈很好的线性关系（r=0．9998），平均回收率为101．28％，RSD=1．71％（n=6）。3批样品中甘草酸的含量以甘草酸铵计，分别为4．042、4．006、4．062mg·g^-1（n=3）。结论 所建方法简便、准确、重复性好，可用于复方白花龙胆的定性和定量分析。

OBJECTIVE To establish the quality standards of Compound Baihualongdan. METHODS The methods of TLC and RP - HPLC were adopted to control the quality. The column was Kromasil C18 （250 mm ×4.6 mm,5 μm）. The mobile phase consisted of methanol- 0. 2 mol·L^-1 ammonium acetate- glacial acetic acid（67 ： 33 ： 1 ）with flow rate of 1.0 ml·min^-1 and the detection wavelength at 250 nm. RESULTS Gentiana purdomii Marq. was identified by TLC. The method displayed good lineafity for glycyrrhizic acid with the range of 0. 402 - 2. 010μg（ r = 0. 9998 ）. The average recovery was 99.66% with RSD of 1.62% （ n = 6）. The contents of three batch samples were 4.042,4.006,4.062 mg·g^-1 ,respectively（n =3）. CONCLUSION The method is sample,aeeurate,reproducible, and suitable for the quality standards in Compound Baihualongdan.