摘要
目的 建立复方白花龙胆的质量标准。方法 采用TLC法定性,RP—HPLC法测定甘草中甘草酸的含量。用Kromasil C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.2mol·L^-1。醋酸铵-冰醋酸(67:33:1)为流动相,流速1.0ml·min^-1,柱温25℃,检测波长250nm.结果 鉴别了复方中的白花龙胆;甘草酸铵进样量在0.402—2.010μg之间,进样量与峰面积呈很好的线性关系(r=0.9998),平均回收率为101.28%,RSD=1.71%(n=6)。3批样品中甘草酸的含量以甘草酸铵计,分别为4.042、4.006、4.062mg·g^-1(n=3)。结论 所建方法简便、准确、重复性好,可用于复方白花龙胆的定性和定量分析。
OBJECTIVE To establish the quality standards of Compound Baihualongdan. METHODS The methods of TLC and RP - HPLC were adopted to control the quality. The column was Kromasil C18 (250 mm ×4.6 mm,5 μm). The mobile phase consisted of methanol- 0. 2 mol·L^-1 ammonium acetate- glacial acetic acid(67 : 33 : 1 )with flow rate of 1.0 ml·min^-1 and the detection wavelength at 250 nm. RESULTS Gentiana purdomii Marq. was identified by TLC. The method displayed good lineafity for glycyrrhizic acid with the range of 0. 402 - 2. 010μg( r = 0. 9998 ). The average recovery was 99.66% with RSD of 1.62% ( n = 6). The contents of three batch samples were 4.042,4.006,4.062 mg·g^-1 ,respectively(n =3). CONCLUSION The method is sample,aeeurate,reproducible, and suitable for the quality standards in Compound Baihualongdan.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2006年第6期585-586,共2页
West China Journal of Pharmaceutical Sciences
基金
四川省青年基金(2006)
四川省应用基础项目(2006)
四川省教育厅项目(No.2005)