HPLC测定半合成品中二乙酰大黄酸的含量

Contents determination of diacetylrhein in semi-synthetic product by HPLC

目的 建立测定二乙酰大黄酸含量的方法。方法 采用Waterssymmetry C18（150mm×4．6mm，5μm）色谱柱。流动相为甲醇-水-冰醋酸（70：30：2），流速0．8ml·min^，检测波长340nm。结果 二乙酰大黄酸的回归方程为：Y=1．17×10^X＋6．02×10^3（r=0．9999），线性范围为0．4391—2．1953μg，回收率为91．1％～96．5％，RSD=1．02％（n=9）。结论 所用方法简便、快速、准确，可用于半合成品二乙酰大黄酸的质量控制。

OBJECTIVE To develop a method for determination of diacetylrhein in semi - synthetic product. METHODS The stationary phase was Waters Symmetry C18 column （150mm × 4.6 nm,5 μm）. The mobile phase was methanol -water- acetic acid（70 ： 30 ： 2 ） with a flow rate of 0.8 ml·min^-1 and the detection wavelength was 340 nm. RESULTS The average recovery of diacetylrhein was 98.3% with RSD of 1.02% （n=9）. The calibration curve of diacetylrhein was linear in the range of 0. 4391 -2. 1953 μg （r= 0. 9999）. CONCLUSION This method is simple, rapid and accurate. It can be applied to quality control of the diacetylrhein in product.