行气坦尼卡尔胶囊中胡椒碱的含量测定

Content determination of piperine in Xingqi Tannikaer capsule by HPLC

目的 建立行气坦尼卡尔胶囊中胡椒碱含量的测定方法。方法 采用HPLC Shimpack CLC C18色谱柱（150mm×4．6mm,0.5μm），甲醇-水（77：23）为流动相，紫外检测波长343nm，外标法计算含量。结果 胡椒碱在0．5～4．0μg·ml^-1范围内线性关系良好（r=0．9998）。平均回收率为99．55％，RSD=1．59％。结论 所建方法准确、可靠，可用于行气坦尼卡尔胶囊的质量控制。

OBJECTIVE To establish a method for determining piperine in Xingqi Tannikaer capsules. METHODS HPLC method was, adopted. Shimpack CLC C18 （ 150 mm× 4.6 mm,0.5 μm） column was used. The mobile phase was methanol - water（77 ： 23） and UV wavelength was 343 nm. External standardization was adopted for calculating the content of piperine. RESULTS HPLC assay was linear in the range of 0.5 - 4.0μg·ml^-1 （r =0. 9998）, The average recovery was 99.55% with RSD of 1.59%. CONCLUSION The method is accurate and reliable. It can be used for quality control of Xingqi Tannikaer capsules.