鸡腺胃型传染性支气管炎病毒的分离与初步鉴定

Isolation and Primary Identification of Avian Proventricular Type Infectious Bronchitis Virus

从陕西省周至县某肉鸡场发生疑似腺胃型传染性支气管炎的鸡群中采集病料,经鸡胚接种分离到1株病毒。电镜观察病毒粒子呈圆形或椭圆形,大小80nm^130nm,具有囊膜和纤突,呈典型冠状病毒形态;该病毒无直接血凝性,经10g/L胰酶处理后血凝价平均高达8log2以上;新城疫病毒干扰试验表明,分离毒能抑制NDV在鸡胚中增殖;人工感染3个品种的1日龄雏鸡35只,其中尼克红鸡很快出现临床症状并死亡,死亡率90%。结合流行病学调查、临床症状及病理变化初步鉴定该分离毒为腺胃型传染性支气管炎病毒。

A strain of virus was isolated from the chickens in Zhouzhi county of Xi＇an which suffered from infectious virus and identified by a systematic assay including electron-microscopic technique, pathohitologic examination, etc. The viron which had capsules and ciliary processes were spherical or circular, about 80 nm-130 nm in diameter. This showed typical characteristics of the coronavirus. The isolated virus had no direct hemagglutination, but could agglutinate erythrocytes and the HA tiler was over 8log2 when it was treated with 10 g/L trypsin. The interference test indicated that the isolated virus could inhibit the multi pliation of NDV in chicken embryo eggs. 35 one-day old chickens of three breeds were infected, Nike red chickens had clinical symptoms and died quickly, the mortality rate 90%. The isolated virus was identified primarily as the avian proventricular type infectious bronchitis virus according to epidemiology, clinical symptom and pathologic characters.