轮状病毒VP4基因的原核表达、蛋白纯化及动物免疫试验

Procaryotic Expression of Rotavirus VP4 Gene,Protein Purification and Animal Immunization

在大肠埃希菌中表达并纯化了轮状病毒SA11株的外壳蛋白VP4,并测定了该纯化产物免疫豚鼠后的中和抗体滴度。结果表明,轮状病毒VP4主要以包涵体的形式存在,利用阴离子交换层析纯化后,VP4纯度为80%,与弗氏佐剂混合接种豚鼠后,所产生的中和抗体效价为1∶500。

Rotavirus SA11 outercapsid protein VP4 was expressed in E. coli, and purified by anion exchange chromatography. The neutralizing antibody titer was calculated after immunization with purified VP4 and adjuvant in Guinea Pigs. VP4 existed as inclusion bodies. After purification, the purity of protein was 80%. The rotavirus specific neutralizing antibody titer was 1 ： 500.