肠源性内毒素血症对重症急性胰腺炎胰腺病理变化的影响

Effects of intestinal endotoxemia on pathological changes of pancreas of severe acute pancreatitis

目的探讨肠源性内毒素血症(IETM)与胰腺病理变化之间的关系。方法将Wistar大鼠40只,雌雄不拘,随机分为假手术(SO)组(8只)和模型组(32只),后者又分为3、12、24和36 h组,每组8只,观察血浆内毒素(ET)和胰腺病理评分的情况。结果血浆ET和血清淀粉酶(AMY)含量于造模后3 h明显升高,与SO组相比差异具有统计学意义(P<0.01),此后各时间点逐渐增高,提示3 h开始有IETM形成,随着IETM水平的升高,胰腺病变程度逐渐加重。结论IETM可促使胰腺病理损害加重。

Objective To investigate the correlation between intestinal endotoxemia （IETM） and pathological changes of pancreas of .severe acute pancreatitis. Methods There were 40 Wistar rats weighing （260± 20）g with no limitation in sexe. They were divide into sham operation （SO） group （n =8） and severe acute pancreatitis （SAP） model group （ n - 32） randomly. The latter was divided into 3-h group, 12-h group, 24-h group and 36-h group randomly （each, n = 8）. The changes of plasma endotoxin and the score of pancreatic pathology was observed. Results There was a significant increase of plasma endotoxin （ET） and serum amylase （AMY） contents 3 h after the modeling. Compared with SO group, they had statistical significance （P〈0.01） 3 h after model establishment, indicating the formation of IETM, and hereafter slowly ascending tendency. With increasing of IETM contents, pathological changes of pancreas were aggravating. Conclusion IETM aggravates pathological changes of pancreas.