溃结饮不同给药途径对溃疡性结肠炎大鼠前炎性细胞因子表达的影响

Different effects of Kuijieyin on expression of inflammatory cytokine in treating ulcerative colitis rat by different administration route

[目的]通过比较口服与局部灌肠给药,探讨不同给药途径治疗溃疡性结肠炎(UC)的抗炎机制。[方法]采用2,4,6-三硝基苯磺酸/乙醇法造模,将大鼠随机分为模型组、奥沙拉嗪钠对照组、溃结饮灌胃组、溃结饮灌肠组和正常组。除正常组外,在正常饲养的同时分别给予蒸馏水、奥沙拉嗪、溃结饮灌胃和溃结饮灌肠;采用免疫组化法及图像分析测定不同给药途径下,血清肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)水平及结肠黏膜TNF-α表达的动态变化。[结果]在抑制机体整体的炎症反应方面,溃结饮灌胃或灌肠治疗均可在急性期发挥作用,在急性期的早期溃结饮灌胃的作用优于灌肠,至后期则灌肠给药下调TNF-α的作用与灌胃给药无显著差异,下调IL-8的作用优于灌胃给药;局部抗炎作用方面,急性期至慢性期溃结饮灌胃或灌肠均有作用,急性期结肠黏膜TNF-α表达灌肠组显著低于灌胃组(P<0.05)。[结论]溃结饮灌肠给药整体抗炎作用的发挥高峰在时序上迟于灌胃给药,在抑制愈后复发方面远期效果优于灌胃给药。局部抗炎作用方面,急性期灌肠的作用优于灌胃给药,至慢性期两者无显著差异。

[Objective]To explore the different antiinflammatory mechanisms of Kuijieyin in the treatment of ulcerative colitis （UC） by oral administration and coloclyster. [Methods] The UC rat model was induced by trinitrobenzene sulfonic acid （TNBS） /ethanol intermixture. All the rats were divide into 5 groups randomly. Normal group and model group were given distilled water; Olsalazine sodium group, Kuijieyin drinking group and Kuijieyin clysters group were treated with dringking olsalazine sodium, drinking Kuijieyin and coloclystering Kuijieyin respectively. Then the changes of TNF-α and IL-8 in serum were observed by ELISA and the expression of TNF-α on the mucous membrane of rectum and colon was detected by immunohisthchemical stain. [Resules] Both drinking and coloclystering Kuijieyin inhibited the whole body inflammation in acute phase. In earlier period of acute stage, drinking Kuijieyin shows more effects than clystering. During the later stage, both administration styles decreased the TNF-α in a similar way, while clystering method down-regulated the IL8 in a better way. From the acute phase to the chronic phase, both have effects on the inhibition of local inflammation. The expression of TNF-α on mucous membrane of coloclystering group in acute phase was lower than that of oral group （P〈0.05）. [Conelusion]Kuijieyin has it＇s effects on the systematical inflammation earlier by oral than by clyster, while Kuijieyin by clyster gives more contribution on inhibiting recur. In acute phase, Kuijieyin inhibited local inflammation better by clyster than by oral, while without any difference in chronic phase.