肝康栓抗鸭乙型肝炎病毒和改善肝脏病理的作用

Inhibitory effects of Gankang suppository on hepatitis B virus and ameliorating hepatic pathology in ducklings

[目的]用鸭乙型肝炎(乙肝)模型研究肝康栓抗鸭乙型肝炎病毒(DHBV)和改善肝脏病理的作用。[方法]用DHBV阳性血清感染1 d龄的樱桃谷雏鸭,制备鸭乙肝模型。将其中36只感染阳性鸭随机分成3组:肝康栓治疗组,0.85%氯化钠模型组和阿昔洛韦(ACV)对照组,每组12只,均连续给药4周。分别于给药前,给药14、28 d和停药7 d时取血清,用实时定量PCR法检测鸭血清中DHBV DNA拷贝数。于停药7 d时,处死各组雏鸭,取肝脏病理切片苏木精-伊红染色后,观察肝脏炎症情况和肝细胞变性程度。[结果]肝康栓治疗组给药14、28 d及停药7 d时鸭血清中DHBV DNA拷贝数的对数值均较给药前降低(均P<0.01)。肝康栓治疗组雏鸭肝脏的肝细胞肿胀率、空泡变率、嗜酸性变率均较同期模型组降低,差异有统计学意义(P<0.05,<0.01)。[结论]肝康栓能有效地抑制鸭体内DHBV DNA的复制,并具有改善肝脏病理的作用。

[Objective]To study the antiviral effect of Gankang suppository （GS） on duck hepatitis B virus （DHBV） in vivo, and observe the changes of hepatic pathology. [Methods} One-dayld Yingtaogu ducklings infected with positive serum of DHBV were used as the hepatitis B ani mal model. Thirty-six positive infected ducklings were randomly divided into 3 groups （n=12, each） and administered continuously for 4 weeks： GS group, 0. 85% saline model group, and acyclovir （ACV） control group. The changes of serum DHBV-DNA copies were detected by the method of real time PCR after the ducklings were treated for 14 days and 28 days, and 7 days after withdrawal. The pathological changes in liver tissue were observed 7 days after withdrawal. [Results]After medicated with GS 14 days, 28 days and 7 days after withdrawal, logarithmic val ue （log） of DHBV-DNA in ducklings＇ sera was obviously lower than that before treatment with significant difference（P〈0. 01）. The hepatocellular hydropic degeneration rate, fatty degenera tion rate and acidophilic degeneration rate in GS group were obviously lower than those of 0.85 saline model group respectively with significant difference（P〈0. 05, P〈0. 01）. [Conclusion] GS could ameliorate hepatic pathology change in DHBV-infected ducklings via inhibiting the replication of DHBV-DNA.