银杏叶提取物对大鼠动脉粥样硬化的防治作用

Expeimental Study of Preventive and Therapeutic Effect of Ginkgo Biloba Extracts (GBe) on Atherosclerosis in Rats

目的探讨银杏叶提取物(EGb)对大鼠动脉粥样硬化(AS)的防治作用。方法将Wistar大鼠随机分为4组:空白组、模型组、叶酸组、EGb组。各组分别予灌胃1%CMC(羟甲基纤维素钠)液2ml、3%高同型半胱氨酸(HHcy)混悬液+1%CMC液各1ml、3%HHcy混悬液+叶酸混悬液各1ml、3%HHcy混悬液+EGb混悬液各1ml,1次/d。8周后,测定各组动物血浆的超氧化物歧化酶(SOD)、丙二醛(MDA)、血栓素B2(TXB2)及6-酮-前列腺素F1(α6-keto-PGF1α)含量变化。结果与空白组比较,模型组血浆6-keto-PGF1α、SOD含量下降,TXB2、MDA含量升高,有显著性差异(P<0.05或P<0.01);与模型组比较,叶酸组和EGb组均可使血浆6-keto-PGF1α、SOD含量升高,TXB2、MDA含量下降,有显著性差异(P<0.05或P<0.01);叶酸组与EGb组间比较,6-keto-PGF1α、SOD、TXB2和MDA含量的差异均无显著性(P>0.05)。结论EGb具有防治和治疗HHcy诱导动脉粥样硬化的作用。

Objective To study the preventive and therapeutic effect of extract of ginkgo biloba （EGb） on atheroselerosis （AS） in rats. Methods 51 male Wistar rats were randomly divided into 4 groups： control group, model group, folic acid group, EGb group. The rats were orally administered in advance in respective group：2 ml 1%CMC, 1 ml 1%CMC＋1ml HHey, 1ml folic acid＋l ml I%CMC, lml EGb ＋1 ml 1%CMC, one time per day. After 8 weeks, the level of plasma SOD, MDA, TXB2 and 6-keto-PGF1α, were observed. Results Compared with the control group, the level of plasma SOD and 5-keto-PGF1α were decreased and the level of plasma MDA and TXB2 were increased significantly （P〈 0.05 or P〈 0.01） in the model group; the level of plasma SOD, MDA, TXB2 and 6-keto-PGF1α had no significant difference （P〉 0.05） in the folic acid group and the EGb group; compared with the model group, the level of plasma SOD and 6-keto-PGF1α were increased and the level of plasma MDA and TXB2 were decreased with significant statistical differences （P〈 0.05 or P〈0.01）; compared with the folic acid group, the level of plasma SOD, MDA, TXB2 and 6-keto-PGF1α have no significant difference（P〉 0.05） in the EGb group. Conclusion The results indicate that EGb can decrease the level of plasma MDA and TXB2, and increase the level of plasma SOD and 6-keto- PGF1α. Therefore, EGb can prevent and treat atherosclerosis induced by HHcy.