益气生骨颗粒剂的提取工艺研究

Study on Extracting Technology of “Yiqi Shenggu Granule”

目的确定益气生骨颗粒剂的最佳提取工艺。方法采用正交实验方法,以黄芪甲苷含量为检测指标,以水提醇沉为提取制备方法,考察各因素的影响。结果最佳工艺如下:加入的水量为10倍,提取次数为3次,提取时间为1h。合并滤液,浓缩至浓度为1.1g/ml,加乙醇使含醇量为60%沉淀,静置18h。结论该法提取效率高,稳定性好。

Objective To determine the optimum extracting technology of ＂Yiqi Shenggu Granule＂. Method The optimum extraction process was selected by the orthogonal test and extraction ratio of astragaloside A was used as a maker and water extraction alcohol precipitation as the preparing technique to investigate the influence of the factors, Results Optimum extracting technology was defined as follows： 10 folds of water, extraction for 3 times and 1 h each time. The mixed decoction was concentrated to 1.1 g/ml and precipitated by adding alcohol to concentration of 60% and laying 18 h. Conclusion The method has high rate of extraction and good stability.