染料木素对前列腺癌细胞恶性增殖的抑制作用

Inhibition effect of genistein on proliferation of prostate carcinoma PC-3 cells

目的研究染料木素(Gen)对人前列腺癌细胞(PC-3)的影响。方法将PC-3细胞在DMEF-12培养液(含10%胎牛血清)中采用开放式单层贴壁培养,采用四甲基偶氮噻唑蓝(MTT)比色法、细胞核分裂指数、集落形成和3H-TdR掺入试验方法进行检测。染料木素剂量设为10,20,40,80μmol/L。以二甲基亚砜(DMSO)为溶剂对照。结果不同剂量的染料木素对PC-3细胞的生长增殖有不同的抑制作用。在MTT试验中,不同浓度染料木素对PC-3细胞的7d抑制率分别为27.7%,58.8%,72.3%,84.4%。在核分裂指数试验中,随着染料木素剂量的增高,其核分裂指数明显降低;遮集落形成试验中,随着染料木素剂量的增加,集落形成抑制作用增加;在3H-TdR掺入试验中,可见随着染料木素剂量的增高,3H-TdR掺入到PC-3细胞中明显的减少,与阴性对照组比较差异有统计学意义。结论染料木素对PC-3细胞的生长增殖有明显抑制作用,可能是染料木素抑制前列腺癌的机制之一。

Objective To study the influence of Genistein on the growth of prostate carcinoma PC- 3 cells in vitro. Methods Prostate cancer cells were grown in DMEF 12 medium supplemented with 10 % bovine serum and 5 ml 10 000 U/ ml of penicillin/streptomycin in an incubator maintained at 5 % CO2 and 37℃. Using cell culture tests（MTT test, index of cellular mitosis, Cloning formation and ^3H - TdR incorporation test）, We examined the growth of PC - 3 cells at four concentrations genistein 10, 20, 40 , 80μmol/L with a negative control （anhydrous alcohol and DMSO v/v）. Results Genistein could inhibied the growth of PC - 3 cells. After treatment with various geinistein concentrations mnentioned above for 7 days, the inhibition rates were 27,7 %, 58,8 %, 72.3 % and 84.4 % respectively. With the increase of genistein concentrations, Mitotic index and Cloning formations were decreased in PC- 3 cells and the quantity of ^3H- TdR incorporation was significantly decreased as compared with the negative control （ P 〈 0.05 ）. Conclusion Genistein can significantly inhibit the growth of PC- 3 cells and it is probably one of the preventive mechanisms of genistein in prostate cancer.