E-钙粘附素基因多态性与胃癌遗传易感性

Relationship between polymorphism of E-cadherin gene and genetic susceptibility of non-cardia gastric cancer

目的探讨E-钙粘附素基因(CDH1)启动子区-160 C→A多态性与胃癌遗传易感性的关系。方法应用以人群为基础的病例对照研究,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测胃癌病例组与健康对照组的-160C→A多态改变的频率。结果CDH1-160C→A多态改变在胃癌病例组中的频率分别是CC型为57.8%、CA型为36.4%、AA型为5.8%,对照组各型频率分别是CC型为58.2%、CA型为34.9%、AA型为6.9%。调整年龄、性别、地区、吸烟、饮酒、饮茶、H.pylori感染以及胃癌家族史等因素后,CDH1各基因型在2组间差异均无统计学意义(χ2=0.29,P=0.87);将CA基因型与AA基因型合并后进行病例组与对照组之间的比较,差异无统计学意义(χ2=0.01,P=0.92)。结论E-钙粘附素基因(CDH1)启动子区-160C→A多态性与胃癌遗传易感性不相关。

Objective To test the hypothesis that a C to A variant in promoter of E-cadherin gene, which is associated with risk of developing non-cardia gastric cancer. Methods Population based case-control study was used and the poiymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） was used to genotype this variant in 207 non-cardia gastric cancer patients and 270 age-sex frepuency-matched cancer free controls. Results The distributions of CDH1 genotypes were not significantly different between cases and controls（χ^2 = 0,29, P = 0.87）, Compared with the CC genotype, the variant CA and AA genotypes were not associated with an increased risk of gastric cancer [ adjusted odds ratio（ORa） were 1.15,0,90 respectively;95% confidence interval（CI） were 0.87- 1.72,0.42 - 2.01 respectively]. Conclusion The C→160a promoter polymorphism of E-cadherin gene may not play a major role in the etiology of non-cardia gastric cancer.