谷胱甘肽纳米Fe_3O_4对人肺腺癌细胞SPC-A1影响

Effect of glutathione modified magnetite on growth of human lung adenocareinoma cell line SPC-A1

目的研究氧化型谷胱甘肽（GSSG）修饰的超顺磁性纳米粒子在体外对人肺腺癌细胞SPC-A1生长的影响。方法分别通过光学显微镜、透射电镜观察加入磁性纳米粒子培养后细胞形貌变化及细胞传代后磁性纳米粒子在细胞质内分布状态。用四甲基偶氮噻唑蓝（MTT）染色法、乳酸脱氢酶（LDH）试剂盒、血细胞计数等方法，测定加入磁性纳米粒子培养后细胞存活率与LDH释放水平变化，测定吸收磁性纳米粒子后细胞生长曲线变化。结果加入含磁性纳米粒子培养液后，细胞形貌没有显著改变，细胞传至6代后胞质内仍存在磁性纳米粒子。培养液中分别加入3种浓度（0．6，1．05，1．5mg／ml）磁性纳米粒子后，细胞存活率均〉90％，细胞LDH释放水平与空白对照相比没有明显改变。内吞磁性纳米粒子后细胞生长曲线没有明显改变。结论在一定浓度范围内（0～1．5mg／ml）。GSSG修饰的超顺磁性纳米粒子对细胞的形貌、存活、传代、生长曲线没有显著影响，显示了较好的生物相容性；透射电镜观察内吞的粒子可以在细胞内保留数代，显示了细胞对纳米粒子的高保留性。GSSG修饰的超顺磁性纳米粒子在生物医学中具有潜在应用价值。

Objective To study the effect of GSSG modified superpararnagnetie particles on the growth of human lung adenocarcinoma cell line SPC - A1 in vitro, Methods Human lung adenocarcinoma cell was cultured in the medium containing GSSG modified magnetic particles, the change of the ceil morphology , was observed through inverted microscopy, the location of GSSG modified magnetic particles in the cytoplasm was observed by transmission electron microscopy. Cell viability was determined by MTT reaction. LDH release level was measured by LDH detection kit. Cell growth curve was measured by using haemacytometer after cell took up magnetic particles. Results The morphology of cell did not change obviously after being cultured with magnetic particles. GSSG modified magnetic particles were located in the cytoplasm after cell subculture six generation. Culturing with three different concentrations of magnetic particles for different time, cell viability was more than 90 percent, LDH release level did not change obviously comparing with the control cell. Cell growth curve did not change greatly after cell uptake magnetic particles. Conclusion At certain concentration range （0 - 1.5 mg/ml）, GSSG modified magnetic particles have no obvious effects on the morphology, viability, subculture and growth curve of cell, magnetic particles have fine biocompatibility. Transmission electron microscopy images show magnetic particles can stay in the cytoplasm after cell subculture several generations, and magnetic particles have high reservation in the cell. GSSG modified magnetic particles have many potential applications in biomedicine.