摘要
目的探讨维生素C(VC)对稳恒磁场致大鼠胚胎中脑神经细胞损伤的保护作用。方法以离体培养的大鼠胚胎中脑神经细胞为研究对象,分别加入浓度为5,10,25,50,100μmol/L的VC后,再置于强度为60 mT的稳恒磁场中作用72 h,以中脑神经细胞的存活率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性为实验指标,反映VC预处理后对稳恒磁场作用致胚胎中脑神经细胞损伤的影响。结果经VC作用过的胚胎中脑神经细胞存活率明显增加,MDA含量减小,SOD、GSH-Px活性增加,并且VC高剂量组效果更明显。结论VC可以提高胚胎中脑神经细胞的抗氧化能力,保护神经中脑神经细胞,减轻稳恒磁场作用所致的胚胎中脑神经细胞的损伤。
Objective To observe the protective effect of VC on damaging embryo midbrain nerve cells after expossure to static magnetic. Methods In vitro cultured embryo midbrain never cells were added VC respective to does（5, 10, 25,50 and 100 μmol/L）resume exposed to intensity 60 raT of static magnetic. After 72 h the survival rate, auperoxide dismutase（SOD） activity, glutathione peroxidase（GSH- Px） activity, and the amount of malondialdehyde（MDA） of embryo midbrain never cells were determined, Results Survival rate, activity of SOD, and GSH - Px activity of embryo midbrain nerve ceils decreased, but MDA concentration of embryo midbrain nerve cells increased after static magnetic effecting, These changes weakened when embryo midbrain nerve cells were exposed to static magnetic after pretreatment with VC. This effect was more significant after pretreatment with VC at the high dose, Conclusion Embryo midbrain never cells can be damaged by static magnetic through peroxidation. VC pretreatment can promote the antioxidatlon and protect embryo midbrain never cells from damages induced by static magnetic.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第12期1481-1482,共2页
Chinese Journal of Public Health
基金
江苏省教育厅自然科学基金资助项自(02KZ030004)
