乙醇对大鼠血脂影响及银杏黄酮的保护效应

Effect of ethanol on rat serum lipid profile and protective effect of ginkgetin

目的研究不同水平慢性乙醇暴露对大鼠血脂水平的动态影响及银杏黄酮对高剂量乙醇所致血脂紊乱的调节效应。方法雄性SD大鼠分别给予乙醇0.8,1.6,2.4 g/kg,高乙醇组预先给予银杏黄酮48或96 mg/kg,另设正常对照组和黄酮对照组。试验第30,60,90 d取尾血测定血清总胆固醇(TC)、甘油三酯(TG)、高密度和低密度脂蛋白胆固醇(HDL-C、LDL-C)水平。结果大鼠摄入低剂量乙醇,TG、TC及LDC-C无明显变化,但HDL-C水平逐渐升高;中剂量乙醇也可升高HDL-C水平,但增幅逐渐下降,且TG、TC水平不断升高;高剂量乙醇暴露后HDL-C呈下降趋势,TG、TC、LDL-C明显升高。对高乙醇组进行银杏黄酮干预,尤其是在高水平时,抑制了HDL-C的下降及TG、TC、LDL-C水平的升高。结论低剂量乙醇摄入增加了血清HDL-C水平,中剂量乙醇摄入后HDL-C的保护效应逐渐丧失,高剂量乙醇则不断加重血脂的异常程度。银杏黄酮对高剂量乙醇所致的血脂紊乱有良好的调节作用。

Objective To study the dynamic response of rat serum lipid profile after chronic ethanol administration with different dose and the regulating effect of ginkgetin on lipid disorder induced by high ethanol. Methods Male SD rats were administrated ethanol at a dose of 0.8, 1.6, and 2.4 g/kg and pretreated by low （48 mg/kg） or high ginkgetin （96 mg/kg）. Serum total cholesterol （TC）, triglyceride （TG）, high and low- density lipoprotein cholesterol （HDL - C and LDL- C） were measured on day 30, 60 and 90 for ethanol - fed or ginkgetin pretreated rats, and compared with the corresponding findings of normal control and ginkgetin control. Results Low ethanol for rats increased serum HDL - C level, but had no substantial influence on other lipid parameters. Medium exposure of ethanol increased TG, TC and HDL content, however, the increasing extent of HDL - C gradually diminished. High intake of ethanol resulted in serum dyslipidemia and the deleterious effect was exacerbated with ethanol exposure duration. Ginkgetin preconditioning inhibited the elevation of TG, TC and LDL - C, and the decrease of HDL - C for high ethanol - fed rats. Conclusion HDL - C concentration is increased in the presence of low ethanol. The protective effect elicited by HDL - C is gradually lost accompanying the decline of its content elicited from medium ethanol regime. High ethanol - induced hyperlipidemia is continuously aggravated with administration. Ginkgetin exerts favorable regulating effect on dyslipidemia provoked by high ethanol.