苯与甲醛致小鼠睾丸细胞DNA损伤联合作用

Combined effect of benzene and formaldehyde on DNA damage in testicle cells of mice

目的探讨苯与甲醛致小鼠睾丸细胞DNA联合损伤效应。方法采用单细胞凝胶电泳方法,分别检测苯染毒(剂量为50,200,800 mg/kg)、甲醛染毒(剂量分别为0.3,1.2,4.8 mg/kg)和苯+甲醛复合染毒后小鼠睾丸细胞DNA损伤情况。结果在受试剂量范围内,小鼠睾丸细胞彗星率随苯或甲醛染毒剂量增加而增加(P<0.01),其彗星尾长随苯染毒剂量增加而延长(P<0.01),随甲醛染毒剂量增加而缩短,尤其高甲醛染毒组(P<0.01);复合染毒组中彗星尾长随联合染毒中苯染毒剂量增加而延长(P<0.01),随联合染毒中甲醛染毒剂量增加而缩短(P<0.01)。苯+甲醛联合作用经析因素分析存在相加作用(P<0.01)。结论苯和甲醛均能彼此增强各自所致的睾丸细胞DNA损伤效应。

Objective To study the combined toxicity effect on the reproducion induced by benzene-formaldehyde. Methods The effect on DNA damage in the testicle cells after peritoneal injection of benzene （50,200,800 mg/kg）, formaldehyde （0.3, 1.2, 4.8 mg/kg） and benzene-formaldehydeat were measured using the single cell gel electrophoresis technique （comet assay） tests respectively. Results Within tested doses, the mumber of comet cells in the testicle cells was significantly increased as benzene of formaldehyde exposue dose increased （ P 〈 0.01 ）. The length of DNA migration was significantly increased as benzene increased （ P 〈 0.01 ） but was shortened as formaldehyde increased, especially high formaldehyde dose （ P 〈 0.01）. Among combined exposure doses, the length of DNA migration was uniformly increased as benzene increased （P 〈 0.01 ） bur was shortened as formaldehyde increased （P 〈 0.01）. There was a addition effect between benzene and formaldehyde with factorial analysis （P 〈 0.01）. Conclusion Benzene and formaldehyde could promote the response of DNA damage of testicular cells each other.