c-erbB-2基因表达与乳腺癌关系的研究

EXPRESSION OF C-ERBB-2,P52 AND PCNA IN BREAST CANCER

目的:探讨乳腺癌组织c-erbB-2 mRNA与蛋白表达之间的关系,从而明确c-erbB-2基因mRNA转录和蛋白表达在乳腺癌演化过程中的意义。方法:建立LightCycler实时RT-PCR方法,检测38例乳腺癌、17例癌旁组织、10例乳腺腺病组织c-erbB-2 mRNA的表达情况;用免疫组化SP法检测以上38例乳腺癌患者c-erbB-2表达情况。结果:乳腺癌组c-erbB-2 mRNA的表达水平明显高于其他两组(均为P<0.001)。c-erbB-2 mRNA表达水平与蛋白表达水平相关(P<0.05)。结论:c-erbB-2 mRNA表达水平的检测有助于乳腺癌的诊断。

Objective： To establish the real time RT- PCR method to measure the expression of c - erbB - 2 mRNA in breast cancer, tissues of beside tumors and tissues of adenosis of mammary glands, to find out the relationship of c- erbB-2 protein with its mRNA. Methods： The real time RT-PCR method for detecting the expression of c- erbB-2 was established successfully. 38 specimens of breast cancer, 17 of beside tumors, 10 of adenosis of mammary glands were detected. Antibodies against c - erbB-2, P53 and PCNA were evaluated by IHC technique for the 38 specimens of breast cancer. Results： There is significant difference in the level of c- erbB-2 mRNA among the three groups （P〈0.05）. The level of c- erbB- 2 mRNA has correlation with the c- erbB-2 protein （P〈0.05）. Conclusion： Detection of c- erbB-2 mRNA can hdp diagnose breast cancer.