三氧化二砷对人肝癌细胞的体外作用及其机制

The effect of arsenic trioxide on human hepatoma cancer cells and its mechanisms in vitro

目的:观察三氧化二砷(As2O3)对人肝癌细胞BEL7402的体外作用及其机制。方法:采用MTT法观察As2O3对BEL7402细胞的生长抑制率;流式细胞仪测定经不同浓度As2O3处理后的BEL7402细胞周期、凋亡相关蛋白bcl-2阳性细胞百分率、增殖细胞核抗原(PCNA)变化。结果:As2O3可显著抑制BEL7402细胞生长,且剂量-效应关系显著(r=0.965 0,P<0.01),半数抑制浓度(IC50)为4.93μmol.L-1;As2O3能显著下调PCNA蛋白表达(P<0.01),并使细胞周期阻滞在G2/M期,但对bcl-2表达无影响(P>0.05)。结论:As2O3可有效抑制人肝癌BEL7402细胞生长,其机制可能与下调PCNA表达及阻滞细胞周期有关。

OBJECTIVE To study the effect of arsenic trioxide （As2O3） on human hepatoma cancer cell line BEL7402 and its mechanisms in vitro. METHODS MTT assay was used to observe the growth inhibitory rate of BEL7402 cells treated with Ass2O3; the cell cycle, apoptosis associated protein bcl-2 and proliferation cell nuclear antigen （PCNA） changes were measured by flow cytometry in BEL7402 cells treated with As2O3. RESULTS As2O3 could inhibit the growth of BEL7402 cells dramatically. There was obvious dosage-effect correlation （r = 0. 965 0, P〈0. 01 ）, its half inhibitory concentration （IC50） was 4. 93μmol· L^-1. As2O3 could decrease PCNA protein expression and induce cell cycle arrest in G2/M phase （P〈0. 01）, but it could not influence bcl-2 expression（P〉0. 05）. CONCLUSION As2O3 could inhibit the growth of BEL7402 cells in vitro dramatically, its mechanisms might be probably associated with the decreased PCNA expression and cell cycle arrest.