马齿苋水提液对衰老小鼠生理机能和肝、脑形态学的影响

Effect of water extract of Purslane herb on physical functions,morphology of hepatic cells and brain neurons in senile mouse

目的:探讨马齿苋水提液对衰老小鼠的生理机能和肝、脑形态学影响。方法:采用小鼠注射D-半乳糖制成衰老小鼠模型,随机将模型小鼠分为高、中、低3个质量浓度组和模型组,另设正常对照组。高、中、低质量浓度组在标准饲料基础上,每日每只分别灌喂2.5,5,10g.mL-1马齿苋水提液0.4mL,饲喂4周。测量各自血清超氧化物歧化酶(SOD)和丙二醛(MDA)含量,图像分析各自肝、脑形态学的改变。结果:高质量浓度组SOD活性水平高于模型组,灌喂马齿苋水提液后各组MDA含量均低于模型组,衰老小鼠脑神经元数与正常对照组小鼠相比有增多趋势并存在剂量效应关系,但没有统计学差异;肝细胞核面积与核浆比均大于模型组,其中核浆比具有统计学差异。结论:马齿苋水提液可能具有改善衰老小鼠生理机能和肝、脑组织形态学改变的作用,并可能具有量效关系。

OBJECTIVE To study the effect of water extract of Purslane herb on physicalfunctions, morphology of hepatic cells and brain neurons in senile mice. METHODS The senile mouse mice induced by D -gal. The senile mice were divided randomly into five groups （one normal control group and three aged experimental groups） and model mice group. Control groups were fed on standard chow and experimental groups were fed on standard chow supplemented with 2. 5,5,10 g·mL^-1 water extract of Purslane herb respectively. SOD and MDA were determined at the end of the 4 th week. The microcosmic images of the hepatic cells and brain neurons using the image-pro plus were also observed. RESULTS SOD levels in the high concentration group were significantly higher （P〈0. 05） than that model mice group, and the levels were not different from that in the young control group MDA level of all water extract of Purslane herb supplemented groups was significantly lower than that of a model mice group （P〈0. 05）. Much more number of brain neurons were observed in the high concentration group than other groups,Brain neurons, hepatic cells and karyons in t high concentration group were bigger than that in other groups （P〈 0. 05）. CONCLUSION Water extract of Purslane herb might play an important role in physical functions, the morphology of brain neurons and hepatic cells in senile mouse. There might have a concentration effect relationship in the process.