非小细胞肺癌中FHIT基因及HER_2表达的意义及相关性研究

The significance and relationship between the expressions of FHIT gene and HER_2 in non-small cell lung cancer

目的探讨在非小细胞肺癌中脆性组氨酸三联体(FHIT)基因及人类表皮生长因子受体2(HER2)表达与临床病理的关系,以及二者在非小细胞肺癌中的相关性。方法用免疫组化法检测50例肺癌组织及21例癌旁非肺癌组织的FHIT基因及HER2阳性表达率。结果肺癌组织中FHIT蛋白表达阳性率为30.00%,显著低于非肺癌组织的80.95%(P<0.05),而且FHIT基因的表达与组织类型、淋巴结转移、TNM临床分期有关。肺癌中存在HER2的过度表达,表达率为56.00%,并与淋巴结转移、TNM分期相关。FHIT基因的表达与HER2存在负相关关系,相关系数(r)=-0.38。结论在肺癌的发生、浸润、转移病变阶段中存在FHIT的表达下调和HER2的过度表达,二者呈负相关。

Objective To examine the expressions of FHIT gene and HEP~ in non - small cell lung cancer（NSCLC）and the relationship between the expressions and the clinieopathological features of NSCLC, and to investigate the relationship between FHIT protein and HER2 expression. Methods FHIT protein and HEP2 expressions in 50 lung cancer cases and 21 adjacent non - cancer tissues were performed by immunohistochemistry. And the positive rates of FHIT and HER2 were measured. Results The positive rates of FHIT protein were 30.00% in lung cancer tissues. The expression levels of FHIT were significandy lower in lung cancer tissues than that in non - cancer lung tissues （ P 〈 0. 005 ） compared with 80.95% in non - cancer lung tissues. The relationships between the expression and histological types, metastatichilar lymphnodes,TNM clinical stage. HER2 were over - expressed in lung cancer in 56.00%. The over - expression of HER2 correlates with the lymph node metastasis, TNM staging. An inverse correlation was found between FHIT protein expression and over - express HER2 （ r = - 0. 38, P 〈 0.05 ）. Conclusions These data indicate that reduction of FHIT gene expression and over - expressed HER2 are frequent in lung cancer. An inverse correlation was found between FHIT protein expression and over - expressed HER2. They may be play an important role in the progression and metastasis of lung carcinoma.