复方灵芝制剂防治化学性肝损伤实验研究

Fufanglingzhi Preparation for preventing and treating chemical liver lesion

目的通过实验操作探讨复方灵芝制剂防治化学性肝损伤的作用。方法分0.33、0.67、2.00g/kg3个剂量组对小鼠每日经口进行灌胃,受试样品为复方灵芝制剂,空白对照组和模型对照组给予蒸馏水。30d后将各组动物禁食16h,模型对照组及各剂量组动物按0.01mL/g一次灌胃给予四氯化碳(CCl4)染毒,各受试组染毒4h后继续给予受试样品。染毒24h后摘取眼球取血,分离血清,测定ALT、AST,同时处死动物,取肝脏进行组织病理学检查。结果模型对照组小鼠的ALT、AST含量比空白对照组高(P<0.05),低、中剂量组的ALT、AST值与模型对照组比较,有下降,差异呈显著性(P<0.05)。结论复方灵芝制剂具有防治CCl4化学性肝损伤的作用。

Objective To investigate the prevention and treatment effect of Fufanglingzhi Preparation on chemical liver lesion by the experiment. Method The experimental mice were divided into 3 groups and given every day different doses of Fufanglingzhi Preparation respectively by intragastrical administration （0.33 g/Kg , 0.67 g/Kg and 2.00 g/Kg ）. The blank control group and model control group were given distilled water respectively. After 30 days the mice in different groups were fasted for 16 hours and then the model control group and 3 Fufanglingzhi Preparation groups were infected with carbon tetrachloride （CCI4） in the dose of 0.01 mL/g intragastrically. These groups were given continuously Fufanglingzhi Preparation in different doses 4 hours after CCI4 infection, and blood samples were taken from eyeballs and serum was separated for determining the contents of ALT and AST 24 hours after CCI4 infection. At the same time the mice were killed and their livers were given histopathological examination. Result The contents of ALT and AST in model control group were higher than that of the blank control group （P〈0.05）. The contents of ALT and AST in the Fufanglingzhi Preparation groups with low-dose and medium-dose were decreased compared with that of the model control group. The difference was significant （P〈0.05）. Conclusion Fufanglingzhi Preparation has the effect of preventing and treating chemical liver lesion induced by CCI4.