免疫组化与粘液组化套染技术的应用

APPLICATION OF COMBINED STAINING TECHNIQUE WITH IMMUNOHISTOCHEMISTRY AND MUCOHISTOCHEMISTRY

建立免疫组化与粘液组化套染技术，同步显示细胞核抗原与粘液成份，研究胃粘膜肠上皮化生与胃癌的关系。结果显示ＰＣＮＡ和ｐ５３阳性均定位于细胞核内，杯状细胞胞浆中硫酸化粘液物质呈蓝色。４６例胃腺癌癌旁组织中，２７例含硫酸化粘液肠化生上皮细胞的ＰＣＮＡ阳性率（６６．７％）显著高于无硫酸化粘液的肠化生细胞（３１．６％）。２１例ｐ５３阳性的胃癌中癌旁组织含硫酸化粘液肠化生的伴随率高于ｐ５３阴性胃癌。实验结果提示含硫酸化粘液肠化生的上皮细胞只有更高的增殖活性，且含硫酸化粘液的肠化生与胃癌细胞ｐ５３异常表达有一定关系。免疫组化与粘液组化套染技术操作简便，结果稳定，色彩鲜明，对比度好，易于观察，在探讨胃粘膜肠化生的组织发生及其与胃癌的关系方面有较好应用前景。

To study the relation between metaplasia of intestinal epithelium of gastricmucous membrane and gastric carcinoma,a combined staining technique with immunohistoche-mistry and mucohistochemistry to display nucleus and mucous component has been applied.Re-sults:positive PCNA and p53 were localized in the nucleus and sulfated substance in gbolet cell cy-toplasm appeared blue colour, Of 46 gastric adenocarcinoma cases, 27 cases which metaplasia ep-ithelium contained sulfated substance showed positive PCNA(66.7%),significantly higher thanthat without sulfated substance(31.6%).21 gastric carcinoma cases with positive p53 had ahigher rate of epithelium metaplasia than those with negative p53.The study shows that metapla-sia epithelium cells contained sulfated substance have higher activity of hyperplasia,and the meta-plaisa is related to expression of p53 of gastric carcinoma. The authors believe that the techniquewill have a good prospect in studying relation between carcinoma for its simplicity,stable result,bright colour,good contrast and easy observation.