寿聪胶囊对老年性痴呆模型大鼠总抗氧化力、脂褐质及脑组织形态学的影响

Influence of Shoucong Capsule on Total Autioxidative Capacity,Lipofuscin and Brain Tissue Pathological Changes in Alzheimer's Disease Rats

目的观察寿聪胶囊对老年性痴呆大鼠脑组织及血清中总抗氧化力(TAOC)、脂褐质(LIP)及脑组织形态学的影响,以进一步探讨寿聪胶囊防治老年性痴呆的作用机制。方法选用Wistar雄性大鼠,随机分成空白对照组、模型对照组、脑复康组及寿聪胶囊低、高剂量组,每组9只。大鼠颈部注射D-半乳糖合并腹腔注射东莨菪碱制备老年性痴呆(AD)大鼠复合模型,应用中药复方寿聪胶囊进行防治,采用分光光度法测定脑组织及血清中TAOC、LIP的含量,光镜下观察脑组织形态学的变化。结果与模型组相比,寿聪胶囊能显著提高AD大鼠脑匀浆及血清中TAOC的活性,降低LIP的含量;而且明显减轻AD的病理损害。结论提示增强脑组织及血清中TAOC活性、降低LIP含量可能是寿聪胶囊防治AD的作用机制之一。

Objective To study the mechanism of Shoucong to prevent and cure Alzheimer＇s disease by observing the effect of shoucong capsule on total autioxidative capacity （TAOC）, Lipofuscin （LIP） in AD rat brain tissue and blood, and brain tissue pathological changes. Mc^ls Male wistar rats were randomly divided into 5 group, blank compared group, model compared group, Naofukang group, Shoucong Capsule （low, high dose） group. AD compound model rats were made by injected with D-galactose on the neck of rats, then injected with scopolamine in the intraperiton of rats. They were prevented and cured with Shoucong Capsule. TAOC, LIP level in rat brain tissue and blood were examined with spectroscopic analysis, brain tissue pathological changes were observed under light microscope. Results Compared with AD model group, Shoucong Capsule enhanced TAOC level in rat brain tissue and blood, deceased LIP level in rat brain tissue, and lessened the pathological injury of dementia rats. Conclusion Enhancing TAOC level and deceasing LIP level in brain tissue and blood may be one of the mechanism for Shoucong Capsule to prevent and cure Alzheimer＇s disease.