HPLC法测定复方苦木消炎片中脱水穿心莲内酯含量

Determination of Dehydroandrographolide in Fufang Kumu Xiaoyan Pian by HPLC

目的建立HPLC法测定复方苦木消炎片中脱水穿心莲内酯的含量。方法以脱水穿心莲内酯为对照,采用高效液相色谱法测定成品含量。色谱柱为Agilent ZORBAX SB-C18(4.6mm×150mm,5μm);检测波长为253nm;以乙腈-0.1%氨水(30∶70)为流动相;流速为1.0mL/min。结果脱水穿心莲内酯在0.04992～4.992μg范围内线性良好(r=0.9999,n=7),平均加样回收率为99.70%,RSD为0.63%(n=9)。结论本方法简单、准确,可用于产品的质量控制。

Objective To establish a HPLC method for the assay of Dehydroandrographolide in Fufang Kumu Xiaoyan Plan. Methods Standard was Dehydroandrographolide, HPLC was used with Agilent ZORBAX SB-C18 column （4.6 mm× 150 mm, 5μm）. The mobile phrase was consisted of acetonitrile-0.1% Ammonia slution （30 ： 70）. The detection wavelength at 253 nm. The flow rate was 1 mL/min. Results The assay displayed good linearity. The linear range of ephedrine Hydrochloride was 0.049 92-4.992 μg （r=0.9999, n=7）. The average recovery was 99.70% and RSD was 0.63% （n=9）. Conclusion This method is simple, accurate and can be used for the quality control of Fufang Kumu Xiaoyan Pian.