摘要
目的建立高效液相色谱法测定链麻喷雾剂中麻黄碱的含量。方法色谱柱:DiamonsilTM C18(200mm×4.6mm,5μm);流动相:0.01mol/L磷酸二氢钠-甲醇-乙腈-三乙胺(600∶320∶80∶1),用磷酸调节pH到3.0±0.2;检测波长:207nm;柱温:30℃;流速:1.0mL/min。结果麻黄碱在0.0312~0.1872mg/mL浓度范围内线性关系良好(r=0.9999);平均回收率为98.94%(RSD=0.8%)。结论该方法快速,准确,可靠。
Objective To establish a method for determination of ephedrin in Lianma Spraying by HPLC. Method The analysis was carried out on C18 column (4.6 mm× 200 mm, 5 μm) by HPLC, the mobile phase was NaH2PO3-CH3OH-CH3CN-C3H15N (600 : 320 : 80 : 1), the flow rate was 1.0 mL/min, the column temperature was 30 ℃, and detective wavelength was 207 nm. Result The linear range of ephedrin was 0.0312-0.1872 mg/mL, the average recovery rate was 98.94%, RSD was 0.8%. Conclusion The method is simple, rapid, accurate and reliable, and can be used to control the quality of the products.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第12期52-53,共2页
Chinese Journal of Information on Traditional Chinese Medicine