摘要
目的观察福辛普利对大鼠心肌缺血/再灌注损伤后细胞凋亡关键酶Caspase-3表达的影响。方法结扎大鼠左冠状动脉前降支,30 m in后松开结扎线,假手术组只穿线不结扎,大鼠随机分为假手术组、缺血/再灌注组、福辛普利组(20 mg/kg)。福辛普利组分别于术前24 h及术前2 h灌胃给药,观察24 h后心肌细胞电镜下的形态改变和Caspase-3 mRNA的表达。结果福辛普利组电镜下肌丝肌节清晰,而缺血再灌注组闰盘结构不清,肌丝断裂,大鼠心肌Caspase-3 mRNA表达灰度值福辛普利组为0.37±0.03,缺血/再灌注组为1.14±0.05(P<0.01)。结论福辛普利可以使心肌缺血/再灌注后的Caspase-3的表达下降。
AIM To study the protective effect of Fosinopril on ischemia and reperfusion rat myocardium and the Caspase-3 expression in myocardium. METHODS Wistar rats were randomly divided into three groups ： Control group （ n = 8） , Ischemia-Reperfusion group （ n = 8 ） , in which the left anterior descending of coronary artery was occluded for 30 min followed by 24 hours reperfusion and Fosinopril （20 mg/ kg） group （n = 8）. Medication was fed through gastric gavage 24 hour and 2 hour before occlusion and the Caspase-3 expression in myocardium was observed. RESULTS The expression of Caspase-3 mRNA in Fosinpril group was less than that of Ischemia-Reperfusion group （0.37 ± 0.03 vs 1.14 ± 0.05 and 0.39± 0.03 vs 1.14 ± 0.05, P 〈 0.01 ） respectively. CONCLUSION Fosinpril can reduce Caspase-3 expression in myocardium, thus protecting ischemia and reperfusion rat myocardium.
出处
《心脏杂志》
CAS
2006年第6期614-616,共3页
Chinese Heart Journal
基金
吉林省科技发展计划资助项目(No.990581-3)
