CD14及Toll样受体4基因多态性与大肠癌的相关性研究

Polymorphism of CDI4 gene promoter but not Toll-like receptor 4 gene is associated with colorectal cancer in Chinese patients

目的探讨我国湖北汉族人Toll样受体(TLR)4基因Asp299Gly和CD14 C-260T基因多态性分布与大肠癌的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测110例大肠癌患者及160例正常对照者TLR4基因Asp299Gly及CD14 C-260T基因型及等位基因频率的分布。结果大肠癌组CD14 C-260T基因型与正常对照组比较,差异有统计学意义(P＜0．05)。正常对照组CC基因型的频率为15．6%,明显低于大肠癌组的31．8%(P=0．0027,OR=0．3968,95%CI=0．2209～0．7129)；正常对照组中CT基因型的频率为48．1%,明显高于大肠癌组的30．9% (P=0．0056,OR=2．074,95%CI=1．246～3．462)。所有样本中均未发现TLR4基因Asp299Gly的突变型。结论CD14 C-260T基因多态性与中国湖北汉族大肠癌显著相关,而TLR4基因Asp299Gly多态性与大肠癌无关。

Objective To investigate an association between Toll like receptor （TLR） 4 Asp299Gly or CD14 C-260T polymorphisms and colorectal cancer in Chinese patients. Methods By a method of polymerase chain reaction-based restriction fragment length polymorphism （PCR-RFLP）, TLR4 Asp299Gly and CD14 C-260T polymorphisms in unrelated 110 patients with colorectal cancer and 160 healthy controls in Chinese Han race population were genotyped. Results Significant differences of CD14 genotypes were found between healthy controls and the patients with colorectal cancer. The frequency of the CC genotype in healthy controls was extremely lower than that in the group of colorectal cancer （15.6% vs. 31.8%, P = 0.0027, OR = 0.3968, 95%CI： 0.2209-0.7129） while the frcquency of the CT genotype in healthy controls was significantly higher than that in the group of colorectal cancer （48.1% vs. 30.9%, P = 0. 0056, OR = 2. 074, 95%CI： 1. 246-3. 452）. No TLR4 Asp299Gly mutant genotype was detected in any patients and healthy controls in Chinese Han population. Conclusion The polymorphism of CD14 C-260T but not TLR4 Asp299Gly is associated with colorectal cancer in Chinese patients.