氯化钆对急性坏死性胰腺炎肺泡巨噬细胞分泌炎症介质的影响

The effect of gadolinium chloride on inflammatory mediators secreted by alveolar macrophages in acute necrotizing pancreatitis rats

目的探讨氯化钆(Gdcl3)对急性坏死性胰腺炎(ANP)肺泡巨噬细胞(AM)分泌炎症介质的影响。方法90只成年SD大鼠随机分为正常对照组(C组)、ANP组、Gdcl3处理正常对照组(C+Gdcl3组)、ANPGdcl3预处理组、ANPGdcl3治疗组。每组18只。各组大鼠均经支气管肺泡灌洗获取肺泡巨噬细胞,行支气管肺泡灌洗液(BALF)中蛋白含量、肺组织髓过氧化物酶(MPO)水平、AM分泌肿瘤坏死因子α(TNF-a)和一氧化氮(NO)水平分析;行血气分析,检测肺湿/干重比值,并行肺组织病理学检查。结果C+Gdcl3组各指标与正常对照组相比差异无显著性(P>0.05)。ANPGdcl3预处理组和ANPGdcl3治疗组除动脉血氧分压外各项指标显著高于正常对照组,差异有显著性(P<0.05),但与ANP组相比有降低,差异有显著性(P<0.05)。结论氯化钆可抑制ANP大鼠AM分泌炎症介质,并减轻ANP所致的肺损伤。

Objective To explore the effect of gadolinium chloride （ Gdcl3 ） on inflammatory mediators secreted by alveolar macrophages （ AMs ） in acute neerotizing pancreatitis （ ANP ） rats. Methods Ninty SD rats were randomly into five groups （ n = 18 in each group ） ： normal control （ C ） group, ANP group, C ＋ Gdcl3 treatment（ C ＋ A） group, ANPGdcl3 pretreatment（AP） group, ANPGdcl3 treatment（AT） group. AMs were obtained by bronchoalveolar lavage. The blood gas the weight ratio of wet/dry lung tissue, the protein content of bronchoalveolar lavage fluids （ BALF ） , the myeloperoxidase （ MPO ） of lung tissue and secretion of TNFa and NO by AMs were evaluated; histologic examination of lung tissue was performed. Results The parameters mentioned above in C ＋ A group compared with C group showed no statistical significance （ P 〉 0.05 ）. Except the blood gas, the indicators mentioned above in AP group and AT treatment group were significantly elevated compared with normal control group （ P 〈 0.05 ）; but lewer significantly compared to the ANP group （ P 〈 0.05 ）. Conclusions Gdcl3 can inhibit the secretion of inflammatory mediators of AMs and ameliorate lung injury of ANP.