MMP-2和MMP-9在视网膜母细胞瘤中的表达及意义

Expressions of MMP-2 and MMP-9 in retinoblastoma and their significance

目的探讨视网膜母细胞瘤(retinoblastoma,Rb)中基质金属蛋白酶-2(matrixmetalloproteinase-2,MMP-2)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达及其与临床病程的关系。方法应用免疫组织化学SP法检测41例Rb组织中MMP-2和MMP-9的表达;用HMIAS-2000型全自动医学彩色图像分析系统测量其平均光密度值。统计学方法比较不同临床和病理阶段Rb表达MMP-2和MMP-9的差异。结果在41例Rb组织切片中均有MMP-2和MMP-9的表达。有视神经浸润的肿瘤MMP-2和MMP-9表达水平明显高于无视神经浸润的肿瘤(P<0.01),处于眼外期的肿瘤MMP-2和MMP-9的表达水平明显高于眼内期和青光眼期的肿瘤(P<0.01)。结论Rb细胞内有MMP-2和MMP-9的表达。MMP-2和MMP-9的表达水平与视神经浸润和临床分期存在密切的关系,提示其表达水平与该肿瘤的浸润与发展有关。

Objective To investigate the expressions of matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （MMP-9） in retinoblastoma （Rb）,and their relationships with tumor development. Methods Immunohistochemical technique was used to detect the expressions of MMP-2 and MMP-9 in 41 cases of paraffin embedded Rb samples. Quantitative analyses of the expressions of MMP-2 and MMP-9 were assessed by HMIAS-2000 Color Pathologic Analysis System. The differences of the expressions of MMP-2 and MMP-9 in each clinical and pathological stage were analyzed statistically. Results Expressions of MMP-2 and MMP-9 were found in all the 41 Rb specimens. The expressions of MMP-2 and MMP-9 were significantly higher in tumors with optic nerve invasion than those In tumors without optic nerve Invasion（P〈 0.01）; and the expressions of MMP-2 and MMP-9 were significantly higher in tumors of extra-ocudar stage than those in tumors of either glaucomatous stage or intra-ocudar stage（P〈0.01）.Conclusion MMP-2 and MMP-9 exist in retinoblastoma cells. The levels of MMP-2 and MMP-9 are related to optic nerve invasion and clinical stage of Rb, which suggests that the expressions of MMP-2 and MMP-9 could be connected to the invasion and development of tumor cells.