短串联重复序列检测法用于同胞血缘关系鉴定

The siblings’ identification with short tandem repeats

目的探讨人类短串连重复序列(STR)基因位点检测技术应用于同胞亲缘关系鉴定的可靠性。方法采集3名已知同胞与1名未知女性个体血样,提取DNA,常规PCR扩增,非变性聚丙烯酰胺凝胶电泳分离,银染显色分析。结果得到具有排除价值的5个位点(D12S375,D8S1179,TH01,FGA和D21S11),否定了未知个体与3名同胞同父同母兄妹的亲缘关系。结论STR基因位点多、多态性高、分布广、灵敏度高、易检测,是法医学个体识别和亲子鉴定的主要技术手段,在同胞鉴定中有较强的适用性。

Objective To study the reliability of genetic profile of 18 short tandem repeats （STR） loci in determining siblings. Methods The DNA was extracted from whole blood of three siblings and a suspicious one. Eighteen short tandem repeats loci were amplified with PCR, which were genotyped with electrophoresis separation on dPAGE. Results Five loci （D12S375, D8S1179, TH01, FGA and D21Sll） were found, by which the suspicious was excluded from the three siblings. The technique of short tandem repeats determination is suitable for the siblings＇ identification.