高糖对脑微血管内皮细胞活力和内皮素-1的影响

Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells

目的:探讨体外培养条件下高糖状态对大鼠脑皮质微血管内皮细胞(Brain microvascular endothelial cells,BMEC)的影响。方法:建立大鼠脑皮质微血管内皮细胞体外培养模型,用透射电镜观察正常浓度(5.05mm ol/L)和高浓度(15mmol/L、30mmol/L)葡萄糖培养基中培养24h后内皮细胞的超微结构变化,用M TT法评价内皮细胞活力,采用放射免疫方法测定内皮素-1(Endothelin-1,ET-1)含量,用原位杂交法测定ET-1mRNA表达。结果:高浓度葡萄糖可使内皮细胞超微结构损伤,细胞活力降低,ET-1分泌显著上调,并呈剂量依赖性损伤细胞,但ET-1 mRNA表达无明显变化。结论:高糖对脑皮质微血管内皮细胞有显著的损伤作用,ET-1升高在糖尿病微血管病变早期可能起主要作用。

Objective ：To investigate the effects of high glucose on rat brain microvascular endothelial cells （BMEC）. Methods： Brain microvascular endothelial cells（ BMEC ）of Wistar rat cerebral cortex were cultured in 5.05mmol/L glucose, 15 and 30mmol/L glucose media respectively for 24h, and then BMEC activity or injury was assayed by observing cell uhramicrostructures morphology and adopting methyl thiazolyl tetrazolium（ MTT）assay.The ET-1 concentrations were evaluated by radioimmunoassay.Situ hybridization used to detect the ET-1 mRNA expression.Results：The BMEC activity and viability assessed by MTT were decreased when glucose concentrations rose .The ul- trastructure of BMEC was damaged and the content of ET-1 in BMEC media was increased. Furthermore, ET-1 mRNA expression was not induced by high concentration of glucose.Conclusions：High concentration of glucose has a significant effect of BMEC injury.The data suggest that ET-1 may play important role in the early state of diabetic microvascular complications.