牙周炎患者TLR2,TLR4基因多态性研究

Study on association between toll-like receptor 2,4 gene polymorphisms and periodontitis

目的:研究TLR2、TLR4基因多态性分布,探讨其与牙周炎的发病和严重程度之间的相关性。方法:选择40位侵袭性牙周炎(AgP)患者及50名慢性牙周炎(CP)患者为实验对象,以100名牙周健康者作为对照组。从全血样本中提取基因组DNA,采用PCR-RFLP方法进行TLR2、TLR4基因多态性分析,使用凝胶电泳和溴乙锭检测等位基因。结果:正常对照组和(AgP)(、CP)患者均为TLR2Arg677Trp基因的杂合型。在AgP组与CP组患者中未发现TLR2Arg753Gln的突变型,而对照组中该基因频率为6%。在所有研究对象中都未发现TLR4Asp299Gly和Thr399Ile的基因多态性。结论:TLR2、TLR4基因的多态性与牙周炎之间无相关性。

Objective： The present study aimed to investigate the relationship between TLRs 2 and 4 gene polymorphisms and periodontitis.Method：40 patients with generalized aggressive periodontitis, 50 patients with chronic periodontitis and 100 periodontally healthy eontrols were reeruited. Genomie DNA was extraeted from whole blood＇samples. TLRs 2 and 4 genes were analyzed by polymerase ehain reaetion-restrietion fragment length polymorphism （PCR-RFLP） with respeetive restrietion endonueleases. The alleles were deteeted by polyaerylamide gel eleetrophoresis and visualized with ethidium bromide. Result. Heterozygosity for the TLR2 Arg677Trp polymorphism was found in all subjects. The TLR2 Arg753Gln mutant allele was not found in periodontitis patients, while a heterozygous frequeney of 6% （6/100） was deteet ed in the eontrols. The TLR4 Asp299Gly and Thr399Ile mutant alleles were not found in any of the subjeets.Conclusion： The present results suggest that TLR2 , 4 gene polymorphism may not be associated with aggressive or ehronie periodontitis.