前列腺癌nm23H1mRNA、TGF-β1mRNA表达及其与肿瘤转移、生存率的相关性研究

STUDY ON THE CORRELATIONSHIP BETWEEN THE EXPRESSION OF nm23H1mRNA, TGF-β1mRNA AND TUMOR METASTASES,SURVIVAL RATE WITH PROSTATE CANCER

应用原位杂交法检测42例前列腺癌组织nm23H1mRNA、TGF-β1mRNA表达,并以CD31抗体为标记,经EnVision^(TM)免疫组织化学及Leica-Qwin计算机图像分析,用Weidner最高血管密度计数法,计数阳性MVD,研究前列腺癌组织nm23H1mRNA、TGF-β1mRNA和CD31的表达与前列腺癌中的新生血管的生成、肿瘤血道转移和调查患者术后的生存率关系。前列腺癌nm23H1mRNA阳性表达66．67%(28例),nm23H1mRNA阳性表达与前列腺癌骨转移、TNM分期、MVD呈负相关(P＜0．05)：TGF-β1mRNA阳性表达78．75%(33例),其与前列腺癌骨转移、TNM分期、MVD呈正相关(P＜0．05),与癌周组织的nm23H1mRNA和TGF-β1mRNA阳性表达比较,具有显著性差异(P＜0．01或P＜0．05)。前列腺癌组织的MVD(78．51±10．29/mm^2)显著高于癌周组织(34．19±9．27/mm^2),两者比较具有显著性差异(P＜0．05)。在根治术后5年内死亡的42．86%(18例)患者中MVD(92．41±15．42/ mm^2),高于5年内生存的患者(62．79±13．58/mm^2),两组间有显著性差异(P＜0．05)；在不同的肿瘤病理学分级中,nm23H1mRNA在前列腺癌未、低分化型中阳性表达率高,高、中分化型中阳性表达率低,两组间有显著性差异(P＜0．05)。当nm23H1mRNA阳性表达率高时,肿瘤骨转移率低,生存率高,故认为nm23H1基因具有抑制前列腺癌发生和转移作用。当TGF-β1mRNA阳性表达率高时,肿瘤骨转移率高,生存率低。故认为TGF-β1基因具有促进前列腺癌发生和转移作用。前列腺癌组织中的MVD与肿瘤骨转移密切相关,前列腺癌组织MVD的显著增高,提示肿瘤组织有新血管的生成。TGF-β1促进了肿瘤诱导的血管新生,在前列腺癌的骨转移中起重要作用。

All the specimens were marked by CD31 antibody, the expression of nm23H1mRNA, TGF-β1mRNA was detected in 42 cases of prostate cancer in situ hybridization with EnVision^TM and Leica-Qwin computer image analysis system. The Weidner＇s highest vessel density counting method was used to analysis the microvessel density（MVD） of the specimens,and the patients＇viability rate after operation was investigated. The correlation between the expression of nm23H1mRNA,TGF-β1mRNA,CD31 and neoangiogenesis, hyperplasia of microvessel, tumor metastases in prostate cancer was studied. The positive expression of nm23H1mRN in prostate cancer was 66.67% （28 cases）. There was negative correlation between the positive expression of nm23H1mRNA and bone metastasizing,TNM staging,MVD in prostate cancer （P〈0.05）. The positive expression of TGF-β1mRNA was 78.75%（33 cases）, and was positive correlative to the bone metastasis, TNM stages, MVD in prostate cancer （P〈0.05）. It is significantly different（P〈0.01 or P〈0.05） with that in adjacent nontumorous tissue. The MVD （78.51±0.29/mm^2） in prostate cancer was significantly （P〈0.05） higher than that in adjacent nontumorous tissue （34.19±9.27/mm^2）. The MVD （92.41±15.42/mm^2） in those who died in five years （18 cases） was significantly higher（P〈0.05） than that（62.79±3.58/mm^2） in those who were still alive. In tumor pathology grading,different differentiation, the difference of nm23H1mRNA positive expression was statistically significant （P〈 0.05）. Higher the nm23H1mRNA expressed,lower the bone metastasized,and higher the viability rate was. Therefore, the nm23H1 gene was thought to have the effect of suppressing prostate cancer occurring and bone metastasizing. Higher the TGF-β1mRNA expressed, higher the tumor bone metastasized, and lower the viability rate was. Therefore, the TGF-β1 gene was thought to have the promote genesis and metastasize of prostate cancer. There is close correlation between the CD31 expression, MVD and prostate cancer bone metastasizing. The higher MVD level indicated neoangiogenesis in prostate cancer. TGF-β1mRNA promote neoangiogenesis induced by tumor and play an important role in bone metastasizing.