cDNA芯片在卵巢癌早诊及临床病理分期中的初步应用探讨

The Studies of Preliminary Application on cDNA Microarray for Early Diagnosis and Clinicopathologic Staging of Ovarian Cancer

目的:探讨PTEN,nm23H1,VEGF165,Tiam1,MMP-2,Timp2,HE4和S100A4基因在卵巢癌发生和侵袭中的作用。方法:采用cDNA芯片技术分别检测20例卵巢癌和5例正常卵巢组织中上述基因cDNA表达谱差异。结果:PTEN,Timp2和nm23H1cDNA在卵巢癌组织中表达下调(CY-3/CY-5<0.5);Tiam1,VEGF165,MMP-2,HE4和S100A4cDNA在卵巢癌组织中表达上调(CY-3/CY-5>2.0);且HE4基因表达上调的最为明显(CY-3/CY-5>5.0)。表达下调和表达上调的上述基因与卵巢癌临床病理分期、组织分化程度关系密切(P<0.01)。结论:上述基因与卵巢癌发生及侵袭关系密切。cDNA基因芯片技术对于探讨卵巢癌分子机制,寻找卵巢癌分子标志物具有重要意义。

Objective： To investigate the roles of tumor suppressor genes-PTEN, nm23H1, VEGF165, Tiam1, MMP-2, Timp2, HE4 and S100A4 in carcinogenesis and progression of the ovarian cancer. Methods： Different expression profiles of the genes in normal ovary （n=5） and ovarian cancer tissue （n=20） were observed by eDNA mieroarray. Results： The PTEN, Timp2 and nim23H1 genes were down-regulated ovarian cancer （CY-3/CY-5〈0.5） and the Tiam1, VEGF165, MMP-2, HE4 and S100A4 genes were up-regulated （CY-3/CY-5〉2.0）. The HE4 gene was up-regulated remarkably （CY-3/CY- 5〉5.0）. Those genes were correlated with clinicopathologic staging of ovarian cancer （P〈0.01）. Conclusions： Those genes are Closely related with carcinogenesis of the ovarian cancer. The cDNA microarray technology will be of significance to the molecular mechanism of carcinogenesis and metastasis of the ovarian cancer and to detection of the marker of specific molecule in the ovarian cancer.