成釉细胞瘤中细胞周期素D1及其抑制因子和hTERT的表达

Expression of Cyclin D1 and Its Inhibitors and hTERT in Ameloblastoma

目的研究端粒酶逆转录酶(hTERT)、细胞周期素D1(cyclinD1)、周期素依赖激酶抑制因子p16INK4、p21WAF1mRNA和p27KIP1蛋白在人成釉细胞瘤(ABs)中的表达。方法用原位杂交和免疫组化SP法分别检测ABs中hTERT、cyclinD1、p16INK4、p21WAF1mRNA和p27KIP1蛋白的表达。结果hTERTmRNA在ABs中有较强表达,51例为阳性表达,cyclinD1mRNA在ABs中23例为阳性表达,而p16INK4、p21WAF1mRNA和p27KIP1蛋白在ABs中分别有17、12、9例为阳性表达。伴随ABs的复发和恶变,hTERT、cyclinD1mRNA阳性率逐渐上升,p16INK4、p21WAF1mRNA和p27KIP1蛋白阳性表达丢失。经Kendall相关性分析得出,hTERTmRNA与p16INK4、p21WAF1mRNA、p27KIP1蛋白之间在ABs中rk分别为-0.587、-0.652、-0.783,均有统计学意义(P<0.001)。结论hTERT在ABs中的活性可能与p16INK4、p21WAF1mRNA、p27KIP1蛋白丢失等多种因子共同调控有关。

Objective To investigate the expression of human telomerase reverse transcripase（hTERT）, cyclin D1 mRNA, p16^INK4, p21^WAF1 mRNA and p27^KIM protein in human ameloblastoma（ABs）. Methods The expression of hTERT, cyclin D1, p16^INK4, p21^WAF1 mRNA and p27^KIM protein in 54 cases of human ABs were detected by in situ hybridization or immunohistochemistry. Results The positive cases of hTERTmRNA, cyclin D1 mRNA was 51, 23, respectively. The positive cases of p16^INK4, p21^WAF1 mRNA and p27^KIM protein was 17, 12, 9. Comparing with recurred and transformed malignantly, the expression of hTERT mRNA, cyclin D1 rnRNA increased, and the expression of p16^INK4, p21^WAF1 mRNA and p27^KIM protein decreased or lost. The expression of hTERT mRNA and p16^INK4, p21^WAF1 mRNA and p27^KIM protein in ABs had middle to high negative relation（rk=-0.587, rk=-0.652, rk=-0.783, P〈0.001）. Conclusion The hTERTmRNA expression in ABs is related to the reguation of p16^INK4, p21^WAF1 mRNA and p27^KIM protein.