摘要
在有低基础血硒层次的一张人口的硒补充被报导了减少包括前列腺癌症的几癌症的发生。基于的在临床的调查结果，至少一 selenoproteins 的抗氧化剂功能为 chemopreventive 效果负责，是可能的，尽管低分子量 seleno 混合物也被安置了有选择地在转变房间导致 apoptosis。在前列腺房间在 selenoprotein 表示上探讨硒补充的效果，我们在 non-tumorigenicprostate 承担了抗氧化剂 selenoprotein 表示以及硒毒性的分析上皮的房间(RWPE-1 ) 和前列腺癌症房间(LNCaP 和 PC-3 ) 。我们的结果证明二个谷胱甘肽过氧化物酶家庭成员(GPX1 和 GPX4 ) 被补加的硒高度在前列腺癌症房间劝诱，但是仅仅稍微在 RWPE-1 导致了房间。另外， GPX1levels 作为与 RWPE-1 或 LNCaP 房间相比在 PC-3 房间是戏剧性地更低的。然而， GPX2 蛋白质 andmRNA 在 RWPE-1 房间仅仅是可检测的。测试的三硒混合物(钠亚硒酸盐，钠硒酸盐和 selenomethionine ) ，仅仅钠亚硒酸盐在硒集中的一个生理的范围显示出毒性。尤其是并且与以前的研究相对照， RWPE-1cells 是显著地对比前列腺癌症的任何一个，房间衬里的亚硒酸盐更敏感。这些结果证明那 selenoproteins 和硒新陈代谢在前列腺房间在多重层次被调整。
Selenium supplementation in a population with low basal blood selenium levels has been reported to decrease the incidence of several cancers including prostate cancer. Based on the clinical findings, it is likely that the antioxidant function of one or more selenoproteins is responsible for the chemopreventive effect, although low molecular weight seleno-compounds have also been posited to selectively induce apoptosis in transformed cells. To address the effects of selenium supplementation on selenoprotein expression in prostate cells, we have undertaken an analysis of antioxidant selenoprotein expression as well as selenium toxicity in non-tumorigenic prostate epithelial cells （RWPE- 1 ） and prostate cancer cells （LNCaP and PC-3）. Our results show that two of the glutathione peroxidase family members （GPX1 and GPX4） are highly induced by supplemental selenium in prostate cancer cells but only slightly induced in RWPE-1 cells. In addition, GPX 1 levels are dramatically lower in PC-3 cells as compared to RWPE- 1 or LNCaP cells. GPX2 protein and mRNA, however, are only detectable in RWPE-1 cells. Of the three selenium compounds tested （sodium selenite, sodium selenate and selenomethionine）, only sodium selenite shows toxicity in a physiological range of selenium concentrations. Notably and in contrast to previous studies, RWPE-1 cells were significantly more sensitive to selenite than either of the prostate cancer cell lines. These results demonstrate that selenoproteins and selenium metabolism are regulated at multiple levels in prostate cells.
