D-柠檬烯对K562细胞增殖及凋亡的影响

Proliferation Inhibition and Apoptosis Induction of K562 Cells by D-limonene

本研究探讨D-柠檬烯对K562白血病细胞增殖的影响及机制。应用MTT试验观察不同浓度D-柠檬烯作用后K562细胞增殖的改变;应用细胞形态学检查、流式细胞术、DNA琼脂糖凝胶电泳观察和检测细胞凋亡。结果表明在0.125-1.0mmol/L浓度范围内,D-柠檬烯对K562细胞增殖的抑制作用呈现剂量依赖的关系。典型的细胞形态学改变、DNA琼脂糖凝胶电泳梯形条带及流式细胞仪检测出亚G1峰共同证实了D-柠檬烯能诱导K562白血病细胞凋亡。结论D-柠檬烯抑制K562细胞的增殖并呈剂量依赖的方式,使细胞滞留于G1期,诱导其凋亡。

This study was aimed to investigate the effect of D-limonene on K562 leukemia cells and its mechamsm. Inhibitory effect of D-limonene on proliferation of K562 leukemia cells was assayed by MTT method and cell apoptosis was detected by flow cytometry and DNA agarose gel electrophoresis, the morphologic change of K562 cells was observed by microscopy. The results showed that when K562 cells were treated with 0. 125 - 1.0 mmol/L of D-limonene for 48 hours, the proliferation of K562 cells was obviously inhibited in dose-dependent manner. Typical morphological changes and the typical DNA ladder on agarose gel electrophoresis for analysis of cellular apoptosis were significantly appeared in D-limonene treated K562 cells. Simultaneously, the sub-G1 peak was found in FCM analysis, It is concluded that the D-limonene can inhibit proliferation of K562 cells in dose-dependent manner, cause cell detained at G1 phase and induce apoptosis of K562 cells.