T-淋巴瘤细胞的超微结构

Ultrastructural Characteristics of Malignant T Cell in T Cell Lymphoma

为了研究T细胞淋巴瘤患者骨髓中恶性T细胞(malignantTcell,MTC)的超微结构,用流式细胞术分析13例T细胞淋巴瘤患者骨髓浸润MTC抗原表达,电子显微镜观察MTC的形态结构。结果发现所有患者MTC大小不等,6/13例细胞大小轻度不均,7例显著不均,直径范围在12-28μm之间。所有患者MTC核异染色质少于正常淋巴细胞,核仁范围在2-8μm之间;10/13例细胞核不规则。8/13例MTC胞浆丰富;7/13例细胞表面有丰富的突起或伪足。5/13例Golgi体、分泌泡、致密颗粒和微丝较丰富;8/13例MTC线粒体肿胀明显。结论骨髓MTC体积普遍不均匀增大;细胞核折叠、切迹、扭曲与核旁微丝增加相关。患者MTC表面突起/绒毛与胞浆Golgi体、分泌泡、致密颗粒及中间微丝呈明显同步发育,大部分患者MTC线粒体明显水肿。

In order to investigate the ultrastructural features of malignant T cell （MTC） in bona marrow aspirate （BMA） from patients with T Cell Lymphoma, the antigen expression of MTC was analyzed by flow cytometry, and the ultrastructural features of MTC in BMA from 13 T-cell lymphoma patients with bone marrow involvement （BMI） were observed by transmission electron microscopy. The results indicated that the sizes of MTC were uneven in every patient and their diameter were between 12 and 28 μm, in 6 out of 13 cases sizes of MTC were slightly uneven but in 7/13 cases sizes of MTC were significantly uneven. The heterochromatin of MTC was less than that of normal T cell and nucleolus diameter was from 2 to 8 μm in all cases. The nuclear contour of MTC was strikingly irregular in 10 out of 13 cases. The MTC had plenty of cytoplasm in 8 out of 13 cases and displayed many microvlli or processes on MTC surface in 7 out of 13 cases, while MTC in 6 out of 13 cases contained more Golgi＇ s apparatuses, secretary vacuoles, dense granules and intermediate filaments. In 8 out of 13 cases mitochondria apparently swelled. It is concluded that the size of MTC increase unevenly in all patients. MTC nuclear contour in most cases is irregular by folding, indenting, and twisting, which often correlated with arising of paranuclear intermediate filaments. Processes and microvilli on surface and Golgig apparatus, secretary vesicles, dense granules as well as intermediate filament in cytoplasm of MTC develop synchronously, meanwhile, mitochondria of MTC strikingly swell in most cases.