抗磷脂综合征识别相关抗原的T细胞克隆受体分析

Characteristics of T Cell Receptors Recognizing Antiphospholipid Syndrome Associated Antigens

为了了解识别抗磷脂综合征相关抗原的T细胞受体特征,用基因谱型图的方法分析一例抗磷脂综合征患者体内的T细胞特征。结果发现优势T细胞呈寡克隆性增生;进一步分析2群主要T细胞克隆的T细胞受体(TCR)基因特征表明,其TCRβ链基因主要由TCRβV8和TCRβV23家族基因编码,T细胞接触抗原的TCRβVCDR3区氨基酸序列分别是CASSLLVAGGPRAYNEQFFGPG和CASSLAGFGQPQHFGDG,其CDR3区模体YNEQFF-GPG和QHFGDG与报道的特发性血小板减少性紫癜和系统性红斑狼疮高度一致。结论这些自身免疫病增殖的T细胞克隆可能识别同样的抗原。

To understand the characteristics of T cell receptors recognizing antiphospholipid syndrome associated antigen, the characteristics of T cells were analyzed using T cell receptor beta variable region （TCRβV） gene spectrotyping in a case of antiphospholipid syndrome （APS）. The results indicated that in the case of APS there were 2 dominant T cell clones. The TCRβVs sequences of the 2 T cell clones showed the TCRβVs belonged to 8 and 23 gene families respectively. The peptides of third complementarity-determining regions （CDR3） in the TCRβVs were CASSLLVAGG-PRAYNEQFFGPG and CASSLAGFGQPQHFGDG. Comparing the motifs in CDR3 with another autoimmune disease, the motif YNEQFFGPG in TCRβV8 and motif QHFGDG in TCRβV23 were identical with that of idiopathic thrombocytopenic purpura and systemic lupus erythematosus reported before. In conclusion, some T cell clones proliferating in these autoimmune diseases may recognize the same antigens.