摘要
在已建立的紫花苜蓿Medicogo sativa体细胞胚高频再生体系的基础上,研究了OD600、侵染时间、负压处理、预培养及共培养时间、脱菌及选择方法等因子对转化效率的影响,并初步获得了抗性体胚。
Several factors （bacterial concentrations, infected time, negative pressure treatment time, pre-culture and co-culture time, acterium free and screening methods etc. ） affecting genetic transfor mation of alfalfa （Medicago sativa）, which is mediated by Agrobacterium tumefaciens with AtNHX1 gene, were studied on the basis of high-frequency regeneration system of alfalfa somatic embryos. Transformation system for alfalfa was successfully set up and the transgenic somatic embryos were also obtained.
出处
《草业科学》
CAS
CSCD
2006年第12期55-59,共5页
Pratacultural Science
基金
国家自然科学基金项目(30671488)
新世纪优秀人才支持计划(NCET-05-0882)
关键词
紫花苜蓿
体细胞胚
根癌农杆菌
ATNHX1
遗传转化
Medicago sativa
somatic embryos
Agrobacterium tumefaciens
AtNHX1
genetic transformation
