摘要
目的观察心肺复苏大鼠心肌组织超微结构变化以及乌司他丁(Ulinastatin,UTI)的影响,探讨心肺复苏大鼠心肌损伤的机制及乌司他丁的治疗机制。方法利用窒息导致大鼠心脏骤停后心肺复苏的动物模型,将Wistar大鼠随机分为3组：对照组(假手术组)、常规复苏组、乌司他丁组,每组8只。于自主循环恢复(ROSC)2h取动脉血,采用酶生化法测定血清中CK-MB的含量；放射免疫法测定血清中TNF-α的含量,采用透射电镜(TEM)观察心肌细胞的损伤情况。结果与常规复苏组比较,乌司他丁组血清中TNF-α的含量显著降低,心功能改善,心肌组织病变减轻。结论大鼠心肺复苏后,血清TNF-α升高参与心肌损伤过程,乌司他丁对复苏后早期的心肌损伤有保护作用。
Objective To study the changes of myocardial ultrastructure and effects of Ulinastatin （UTI） on rats after cardiopulmonary resuscitation （CPR） , and to investigate their mechanism. Methods Wistar rats were randomly divided into three groups： sham control group ; routine treatment group; UTI treatment group （ n = 8, per group ）. Cardiac arrest was induced by asphyxiation in groups except A and CPR was performed. Two hours after recovery, arterial blood was drew for determination of serum TNF-α and CK - MB. The uhrastructural damages were observed with transmission electron microscope （TEM）. Results Compared with routine treatment group, serum level of TNF-α in UTI treatment group decreased significantly and heart function improved. The damage of myocardial ultrastructure was ameliorated markedly. Conclusions TNF-α is probably the extremely important factors that promote the myocardial injury. Ulinastatin can improve the heart function of rats during early stage of resuscitation, and may alleviate the myocardial injury.
出处
《中国急救医学》
CAS
CSCD
北大核心
2006年第12期920-922,共3页
Chinese Journal of Critical Care Medicine
基金
北京市教育委员会科技发展计划项目(No.KM200510025009)
关键词
心肺复苏
心肌超微结构
乌司他丁
Cardiopulmonary resuscitation （CPR）
Myocardial ultrastructure
Ulinastatin
