反相离子对高效液相色谱法检测肾上腺素与肾上腺酮

Analysis of adrenalin and corticosterone in ion-pair reversed-phase high-performance liquid chromatography

采用反相离子对高效液相色谱法测定肾上腺素与肾上腺酮．流动相为甲醇：25mmol／L醋酸钠-醋酸缓冲液（15：85，V／V），庚烷磺酸钠（5mmol／L）为离子对试剂，流速1．0mL／min，检测波长233nm，柱温25℃．在肾上腺素与肾上腺酮浓度分别为36-1800mg／L，36～1350mg／L范围内，其质量浓度与峰面积呈现良好的线性关系（r=0．9998，0.9995）．当信噪比（S／N）为3时，二者的最低检测浓度分别为1．4和2．8mg／L．该法灵敏度高，可用于肾上腺素与肾上腺酮的定量分析．

Adrenalin and corticosterone were determined by an ion - pair reversed - phase chromatography with a mobile phase composed of methanol and 25 mmol/L acetate buffer （ contained 5 mmol/L sodium octanesulfonate, pH 4.7 ） （ 15：85 V/V）. The operation was performed at the flow rate of 1.0 mL/min, and UV detection wave - length was 233nm. The results showed that the peak area and concentration showed excellent correlation, when mass concentration of adrenalin and corticosterone were 36 - 1 800 mg/L and 36 - 1350 mg/L （ r = 0.999 8,0.999 5） respectively. The detection limits of both adrenalin and corticosterone were 1.4 and 2.8 mg/L, if the rate of signal/noise was 3. All these indicated the method is suitable to analyze the adrenalin and corticosterone in broad areas.