摘要
目的了解白细胞介素(IL)1-β31C/T多态性在重庆地区慢性胃炎患者中的分布情况,探讨IL-1β基因多态性与重庆地区胃幽门螺杆菌感染的关系。方法收集重庆地区168例慢性胃炎患者及150例健康对照人群的外周血标本,提取DNA,用聚合酶链反应限制性片段长度多态性法(PCR-RFLP)检测IL-1-β31C/T多态性基因及基因型频率情况,采用快速尿素酶法检测胃组织的幽门螺杆菌感染情况,比较慢性胃炎患者组和健康对照组-31C/T基因及基因型频率的分布差异以及慢性胃炎IL-1β-31C/T不同基因型患者幽门螺杆菌感染率。结果IL-1-β31T等位基因在慢性胃炎和健康对照组的分布频率分别为52.6%和48.1%,慢性胃炎组略高于健康对照组,但差异无统计学意义(P>0.05);慢性胃炎患者组中,IL-1-β31T/T-、31C/T及-31C/C基因型胃幽门螺杆菌感染率分别为92.00%、90.67%及79.07%,IL-1-β31T/T纯合子型和T/C杂合子的胃幽门螺杆菌感染率显著高于-31C/C基因型(P<0.05)。结论IL-1β-31T等位基因增加胃幽门螺杆菌感染的风险性,IL-1β-31T/T基因型可能与重庆地区胃幽门螺杆菌感染发生风险有关。
Objective To investigate the frequence of interleukin-1β(IL-1β) -31 C/T single nucleotide plymorphism (SNP) in chronical gastritis patients in Chongqing district, The relationship between IL-1β -31 SNP and Helicobacter pylori (Hp) infection was also explored. Methods One hundred and sixty-eight gastritis patients and one hundred and fifty healthy Chongqing people were enrolled in tiffs study. Peripheral blood DNAs were extracted by the standard proteinase K digestion and phenol/chloroform method. Polymorphism at -31 locus ( C to T) of IL-1β was genotyped with the method of restriction fragment length polymorphism (RFLP) . Hp infection was detected by the rapid urea enzyme reaction. The gene and genotype frequenees of IL-lβ -31 SNP between gastritis patients and control group were compared. The Hp infection rates between different genotypes at -31 locus were also compared. Results The IL-1β-31 SNP frequence in the gastritis patients and control group were 52.6% and 48.1%, respectively. The frequence in gastritis patients group was slightly higher than that in control group. The Hp infection rates in T/T, T/C and C/C genotypes were 92.02%, 90.67% and 79.07%. higher than that in C/C genotype (P〈0. 05). Conclusion The Hp infection rates in the former two genotypes were significantly -31T allele of IL-1β might increase the risk of Hp infection.
出处
《重庆医学》
CAS
CSCD
2006年第23期2122-2123,共2页
Chongqing medicine
关键词
白细胞介素1Β
基因多态性
胃
幽门螺杆菌
interleukin-lβ
single nueleotide polymorphism
gastritis
Helieobacter pylori