G蛋白β3亚单位基因C825T多态性与蒙古族原发性高血压的相关性研究

The association research between C825T polymorphism of G proteinums β3 subunit gene and Mongolian patients with essential hypertension

目的探讨G蛋白β3亚单位基因C825T多态性与蒙古族人群原发性高血压患者之间的关系。方法采用Sequenom系统检测分析方法检测124例健康人和143例高血压患者的G蛋白β3亚单位基因C825T多态性。结果（1）蒙古族人群GNB3基因C825T位点CC、CT、TT基因型频率在高血压组和正常血压组分别为0．48、0．41、0．11和0．43、0．47、0．10，差异无显著性（χ^2=0．162，P=0．688；OR：1．176，95％CI 0．533～2．592）；C、T等位基因频率在高血压组和对照组分别为0．69、0．31和0．67、0．33差异无显著性（χ^2=0．094，P=0．759；OR：0．945，95％CI0．657—1．358）。（2）蒙古族人群GNB3基因C825T位点CC、CT、TT基因型频率在单纯收缩压增高组和正常血压组分别为0．57、0．35、0．08和0，43、0．47、0．10．差异无显著性（χ^2=0．733．P=0．392；OR：1．957，95％CI0．623—6．143）；C、T等位基因频率在单纯高血压组和对照组分别为0．74、0．26和0．67、0．33，差异无显著性（χ^2=2．133，P=0．144；OR：0．697，95％CI0．428—1．133）。结论G蛋白β3亚单位基因C825T位点与蒙古族人群原发性高血压的发生可能无关，不是蒙古族人群原发性高血压的遗传标志．

Objective ： To analyze the association between C825T polymorphisms of G proteinum＇s β3 subunit gene and Mongolian patients with essential hypertension （EH）. Methods： Sequenom system was used to test G proteinum＇s β3 subunit gene C825T polymorphisms of 124 healthy aduhs anti 143 patients with EH. Results ： （1） The CC , CT, TT genetype frequencies of G proteinum＇s β3 subunit gene C825T site were 0. 48, 0.41, 0. 11 in mongol nation patients with EH and 0. 43, 0.47, 0. 10 in healthy adults, there were not significent difference in two groups （χ^2 =0. 162, P =0. 688; OR： 1. 176, 95% CI 0. 533 -2. 592） ; the frequencies of C, T allele were 0. 69, 0. 31 in patients groups and 0. 67, 0. 33 in healthy groups, there were not significent difference in two groups too （χ^2 =0.094, P =0.759; OR： 0.945, 95% C10.657-1.358）. （2） The CC, CT, TT genetype frequencies were0.57, 0.35, 0.08 in simple high contractive pressure groups and 0. 43, 0. 47, 0. 10 in normal pressure groups, two groups had no significent difference （χ^2 = 0. 733, P = 0. 392 ; R ： 1. 957, 95% CI 0. 623 - 6. 143） ; the frequencies of C, T allele were 0. 74, 0. 26 in simple high contractive pressure groups and 0. 67, 0. 33 in normal pressure groups, two groups had no significent difference too （χ^2 = 2. 133, P =0. 144; OR： 0. 697, 95% CI 0. 428 - 1. 33）. Conclusion： C825 T site of G proteinum＇s β3 subunit gene may be not related to Mongolian patients with EH, it was not genelic mark of EH.