压电免疫传感器用于抗丙型肝炎病毒抗体检测的实验研究

Research on piezoelectric immunosensor for detection of antibody to hepatitis C virus

目的研制一种用于检测抗丙型肝炎病毒(HCV)抗体的压电免疫传感器。方法采用聚乙烯亚胺黏附、戊二醛交联的方法,选用(HCV)人工合成多肽抗原作为生物敏感材料,研制成一种新型的抗HCV抗体压电免疫传感器,将其用于临床血样的检测,探索了传感器的组装条件,考察了传感器的响应特性。结果HBsAg对抗HCV抗体检测干扰较小,且抗HCV抗体阳性血清的响应值与阴性血清的噪声值之比大于4。结论该方法选择性好,易于操作,能快速有效地检测抗HCV抗体。

Objective To develope a new kind of piezoelectric immunosensor which could detect anti-HCV antibody in short time. Methods The corresponding sensitive material was fixed on the piezoelectric immunosensor by polyethylenimine adhesion and glutaraldehyde cross-linking method. The assembly condition and response characters were investigated and the piezoimmunosensor could be used in detection of clinical blood specimen. Results The respond of the positive serum was 4 times higher than that of negative serum. HBsAg had little interference to the detection of anti-HCV antibody. Conclusion The piezoelectric immunosensor prepared in this study is highly selective,easy to be operated for effective detection of anti-HCV antibody.