肺结核患者血浆中热休克蛋白70的表达及意义

Expression and significance of heat shock protein 70 in pulmonary tuberculosis patients

目的通过检测肺结核患者外周血中热休克蛋白70(HSP70)的表达情况,旨在探讨HSP70与肺结核的关系。方法采用双抗体夹心ELISA法检测32例活动性肺结核组患者外周血中HSP70蛋白的表达情况,并与25例稳定性肺结核组及30例健康对照组进行对照分析。结果活动性肺结核患者血浆中HSP70蛋白表达水平为(7.5±1.3)ng/ml,显著高于稳定性肺结核组(5.5±1.7)ng/ml及健康对照组(5.3±0.8)ng/m(P<0.01);稳定性肺结核组与健康对照组比较差异无显著性(P>0.05)。结论活动性肺结核患者存在HSP70的过度表达,提示HSP70可能通过某些未明机制参与活动性肺结核的发病,并可辅助肺结核活动性的判断。

Objective To determfine the expression and signitleance of heat shock protein 70 （HSP70） in pulmonary tuberculosis （TB） and explore the relationship between HSP＇/O with TB. Methods The method of sandwich EIJSA was used to detect the expression of HSP70 in the blood plasma from 32 pulmonary TB patients which was compared with 25 stable pulmonary TB patients and 25 healthy controls. Results The expression of HSP70 in the blood plasma in active pulmonary TB patients, stable pulmonary TB patients and healthy controls was （7.5±1.3） ng/ml, （5.5± 1.7）ng/ ml and （5.3± 0.8）ng/ml respectively. Active pulmonary TB patients had higher level of HSP70 expression in blood plasma compared with healthy controls and stable pulmonary TB patients （P 〈0.01）. There was no significant difference between stable pulmonary TB patients and healthy controls （P 〉0.05）. Conclusions There is over- exprcssion of HSP70 in active pulmonary TB patients and it could be took as one of the diagnosis marks of active pulmonary TB.