摘要
目的:建立以α_1-酸性糖蛋白柱(α_1-AGP)为手性同定相的高效液相色谱法直接拆分雷贝拉唑对映体。方法:研究了流动相中有机改性剂的种类和含量、流动相的 pH、缓冲溶液的浓度和流速等对拆分的影响。结果:以乙腈-0.02 mol·L^(-1)磷酸盐缓冲液(pH 6.0)(14:86)和甲醇-0.02 mol·L^(-1)磷酸盐缓冲液(pH 6.0)(20:80)为流动相,流速为0.8 mL·min^(-1),在5min 内实现了雷贝拉唑对映体在α_1-酸性糖蛋白柱上的拆分。结论:流动相中有机改性剂的种类和比例、流动相的 pH、缓冲溶液的浓度是影响拆分的重要因素。
Objective:To establish an HPLC method to separate rabeprazole and its enantiomer on α1-acid glycoprotein chiral column. Method: The effects of chromatographic conditions including pH of mobile phase, ionic strength,type and concentration of organic modifiers, flow rate on chiral separation were studied. Results: Rabe- prazole enantiomers were separated using an acetonitrile - 0. 02 mol ·L^-1phosphate buffer( pH 6. 0) ( 14: 86) and a methanol -0. 02 mol ·L^-1 phosphate buffer (pH 6.0) (20: 80) at 0. 8 mL·min^-1 Conclusion:The pH of mobile phase, ionic strength, type and concentration of organic modifiers,flow rate on chiral separation were important chromatographic variables in the separation of rabeprazole enantiomers.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第12期1715-1717,共3页
Chinese Journal of Pharmaceutical Analysis